使用新装置制备蛋白质和RNA的聚丙烯酰胺-琼脂糖凝胶电泳。
Preparation polyacrylamide-agarose gel electrophoresis of proteins and RNAs by the use of new device.
作者信息
Asao T
出版信息
Prep Biochem. 1979;9(3):303-20. doi: 10.1080/00327487908061694.
PMID:471945
Abstract
Quantitatively reproducible results were obtained by using a new device for preparation gel electrophoresis combined with polyacrylamide-agarose composite gel. When an adequate gel-buffer system was selected according to the procedure described in this paper, proteins and RNA's were well separated and recovered. The new device for preparative gel electrophoresis and the method for preparation of polyacrylamide-agarose composite gel are presented together with the elution profiles of the recovered substances.
摘要
通过使用一种用于制备凝胶电泳的新装置与聚丙烯酰胺 - 琼脂糖复合凝胶相结合，获得了定量可重复的结果。当根据本文所述的程序选择适当的凝胶缓冲系统时，蛋白质和RNA能得到很好的分离和回收。本文介绍了制备凝胶电泳的新装置、聚丙烯酰胺 - 琼脂糖复合凝胶的制备方法以及回收物质的洗脱曲线。
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