Quarfordt S H, Greenfield M F
J Clin Invest. 1973 Aug;52(8):1937-45. doi: 10.1172/JCI107378.
The kinetics of cholesterol and bile acid turnover were determined from an analysis of the biliary lipids after a single intravenous injection of labeled cholesterol. A compartmental model was designed for the system, and the fractional metabolic rates and fluxes were determined in one lean and two obese normal humans. Each of the normals converted about 3% per day of their rapidly miscible cholesterol pool to cholic acid and 1% per day or less to chenodeoxycholate. Cholate was catabolized at about twice the rate of the dihydroxy bile acids in these normals. Two of the normals were fed corn oil with little change in their kinetic parameters from the control state. The other normal received cholestyramine and dramatically increased the bile acid flux with little change in neutral sterol catabolism. A cirrhotic patient was also studied by this technique and noted to have kinetic parameters quite different from the normals.
通过单次静脉注射标记胆固醇后对胆汁脂质的分析，测定了胆固醇和胆汁酸的周转动力学。为该系统设计了一个房室模型，并在一名瘦人和两名肥胖正常人中测定了分数代谢率和通量。每个正常人每天将其快速混合的胆固醇池的约3%转化为胆酸，每天将1%或更少转化为鹅去氧胆酸。在这些正常人中，胆酸的分解代谢速率约为二羟基胆汁酸的两倍。其中两名正常人摄入玉米油后，其动力学参数与对照状态相比变化不大。另一名正常人接受消胆胺治疗，胆汁酸通量显著增加，中性固醇分解代谢变化不大。还通过该技术对一名肝硬化患者进行了研究，发现其动力学参数与正常人有很大不同。
