Askenase P W
Immunology. 1973 Jul;25(1):47-53.
Normal mouse peritoneal exudate cells (PEC), consisting of 90–95 per cent macrophages, were used to augment haemagglutination in microtrays. Antibody titres were augmented 1000-fold when 10 PEC/ml were mixed with sheep erythrocytes (SRBC) and added to serial dilutions of mouse anti-SRBC sera. This method has certain advantages over conventional antiglobulin augmented haemagglutination. It is thought that augmented haemagglutination by PEC is mediated by macrophage receptors for cytophilic antibodies or by a non-specific surface mechanism.
正常小鼠腹腔渗出细胞(PEC),其中90%-95%为巨噬细胞,用于增强微量培养板中的血凝反应。当每毫升10个PEC与绵羊红细胞(SRBC)混合,并加入小鼠抗SRBC血清的系列稀释液时,抗体效价可提高1000倍。该方法比传统的抗球蛋白增强血凝反应具有某些优势。据认为,PEC增强血凝反应是由巨噬细胞对嗜细胞抗体的受体介导的,或是由一种非特异性表面机制介导的。