公羊睾丸和附睾中的N-乙酰己糖胺酶成分。
The N-acetylhexosaminidase components of the ram testis and epididymis.
作者信息
Bullock S, Winchester B
出版信息
Biochem J. 1973 Jul;133(3):593-9. doi: 10.1042/bj1330593.
Abstract
Three and four N-acetylhexosaminidase components, from ram testis and epididymis respectively, have been separated by ion-exchange chromatography on DEAE-cellulose. Although they all have the same molecular weight (approx. 140000) and very similar catalytic properties towards the synthetic substrates, 4-methylumbelliferyl N-acetyl-beta-glucosaminide and N-acetyl-beta-galactosaminide, isoelectric focusing of the individual components showed that each had a distinct pI value. Isoelectric focusing has also been used to demonstrate the occurrence of multiple forms in ejaculated ram semen.
摘要
通过在DEAE-纤维素上进行离子交换色谱法，分别从公羊的睾丸和附睾中分离出了三种和四种N-乙酰己糖胺酶组分。尽管它们的分子量均相同（约140000），并且对合成底物4-甲基伞形基-N-乙酰-β-葡萄糖胺和N-乙酰-β-半乳糖胺具有非常相似的催化特性，但对各组分进行等电聚焦显示，每种组分都有一个独特的pI值。等电聚焦也已用于证明射出的公羊精液中存在多种形式。
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本文引用的文献
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