通过等电聚焦法比较公羊睾丸和附睾中的N-乙酰己糖胺酶成分。
Comparison of the N-acetylhexosaminidase components in the ram testis and epididymis by isoelectric focusing.
作者信息
Winchester B G
出版信息
Biochem J. 1971 Oct;124(5):929-34. doi: 10.1042/bj1240929.
Abstract
- Several enzymic components, with both N-acetyl-beta-glucosaminidase and N-acetyl-beta-galactosaminidase activity, have been demonstrated in the ram testis and epididymis by isoelectric focusing. 2. The component (I), which predominates in the testis and caput of the epididymis, is isoelectric at pH6.2+/-0.1, whereas the predominant component (II) in the epididymal isthmus and cauda is isoelectric at pH7.0+/-0.1. 3. The total activity and the relative proportions of the enzymic components vary in the different sections of the epididymis. 4. Although their pH optima are slightly different, the appropriate K(m) values for components I and II for the hydrolysis of 4-methylumbelliferyl-N-acetyl-beta-glucosaminide and N-acetyl-beta-galactosaminide and the ratios of the maximal velocities towards the two substrates are very similar.
摘要
- 通过等电聚焦已在公羊的睾丸和附睾中证实了几种具有N-乙酰-β-氨基葡萄糖苷酶和N-乙酰-β-半乳糖苷酶活性的酶成分。2. 在睾丸和附睾头部占主导的成分（I）在pH6.2±0.1时达到等电点，而在附睾峡部和尾部占主导的成分（II）在pH7.0±0.1时达到等电点。3. 酶成分的总活性和相对比例在附睾的不同部位有所不同。4. 尽管它们的最适pH值略有不同，但成分I和II水解4-甲基伞形酮基-N-乙酰-β-氨基葡萄糖苷和N-乙酰-β-半乳糖苷的合适K(m)值以及它们对两种底物的最大速度之比非常相似。
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