对2,4-二硝基苯基基团具有特异性的天然抗体。
Natural antibodies specific to the 2,4-dinitrophenyl group.
作者信息
Farah F S
出版信息
Immunology. 1973 Aug;25(2):217-26.
Abstract
The immunoadsorbent 2,4-dinitrophenyl-amino-ethyl cellulose (DNP—AEC) was prepared by the reaction of dinitrofluorobenzene and aminoethyl cellulose in an alkaline medium. It was found to be an insoluble stable complex, specific for dinitro-phenyl antibody. The immunoadsorbent specifically retained a protein fraction (Fr. C) from pooled and individual human γ-globulins. This fraction was shown to behave like anti-DNP antibody when tested by equilibrium dialysis, fluorescent quenching and spectral shift analysis. The antibody activity was found to reside in the Fab fragment. The significance of finding naturally occuring anti-DNP antibodies in normal serum is discussed.
摘要
免疫吸附剂2,4 - 二硝基苯基 - 氨基乙基纤维素（DNP - AEC）是通过二硝基氟苯与氨基乙基纤维素在碱性介质中反应制备的。它是一种不溶性稳定复合物，对二硝基苯基抗体具有特异性。该免疫吸附剂从混合的和个体的人γ - 球蛋白中特异性保留了一个蛋白质组分（组分C）。当通过平衡透析、荧光猝灭和光谱位移分析进行测试时，该组分表现得像抗DNP抗体。发现抗体活性存在于Fab片段中。讨论了在正常血清中发现天然存在的抗DNP抗体的意义。
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