Jones V E
Immunology. 1969 May;16(5):589-99.
Two antigenically distinct populations of rat antibodies to the 2,4-dinitrophenyl group (DNP) have been isolated by chromatography on DEAE-cellulose and named fraction 1 and fraction 3. Both fraction 1 and fraction 3 had sedimentation coefficients of 6.9S, similar hexosamine contents (1.04 and 0.89 per cent) and electrophoretic mobilities similar to the γ- and γ-immunoglobulins of guinea-pigs and mice. Fraction 1 consisted of two distinct antibody populations with very similar mobilities. Only fraction 1 was able to sensitize homologous rat skin for passive cutaneous anaphylaxis (PCA). Both preparations were active in haemolysis of passively sensitized sheep red cells in the presence of complement, and fraction 3 was more lytic than fraction 1. In other tests such as PCA in mice, passive haemagglutination and precipitation in gel the behaviour of the two fractions was similar. Fraction 3 is probably the immunoglobulin designated γA in earlier reports. The results indicate that fraction 1 and fraction 3 correspond to the 7S γ- and 7S γ-immunoglobulins of guinea-pigs and mice, except for their behaviour in homologous PCA and in passive haemolysis, and that fraction 1 and fraction 3 are rat 7S γ- and 7S γ-immunoglobulins, respectively.
通过在二乙氨基乙基纤维素上进行层析，已分离出两种对2,4 - 二硝基苯基（DNP）具有抗原性差异的大鼠抗体群体，分别命名为组分1和组分3。组分1和组分3的沉降系数均为6.9S，己糖胺含量相近（分别为1.04%和0.89%），电泳迁移率与豚鼠和小鼠的γ - 及γ - 免疫球蛋白相似。组分1由两个迁移率非常相似的不同抗体群体组成。只有组分1能够使同源大鼠皮肤对被动皮肤过敏反应（PCA）致敏。在补体存在的情况下，两种制剂对被动致敏绵羊红细胞的溶血均有活性，且组分3的溶血活性比组分1更强。在其他试验中，如小鼠的PCA、被动血凝和凝胶沉淀试验，两种组分的表现相似。组分3可能就是早期报告中命名为γA的免疫球蛋白。结果表明，组分1和组分3分别对应于豚鼠和小鼠的7Sγ - 及7Sγ - 免疫球蛋白，但在同源PCA和被动溶血试验中的表现除外，并且组分1和组分3分别是大鼠的7Sγ - 和7Sγ - 免疫球蛋白。
