Pkhakadze G A, Galatenko N A, Lipatova T Z, Iatsenko V P, Konoplitskaia K L
Ukr Biokhim Zh (1978). 1979 Jul-Aug;51(4):355-62.
The growth of fibroblasts of the rat subcutaneous tissue was studied as affected by different doses of trypsin, free and bound, on polyurethane substrates. The method of tissue culture provides essential criteria for estimating the degree of bioincompatibility of polymeric alloimplants in particular when immobilizing biologically active substances on them. Trypsin adsorbed on the polyurethane substrate diffusing gradually into the culture medium is established to inhibit the cell growth. Chemically bounded trypsin in the composition of the polymeric matrix at the early stages of cultivation stimulates for a long time the division of fibroblastic elements and further accelerates their degeneration. It is shown that on polymeric substrates containing no trypsin, the growth character and dynamics of the fibroblastic elements are similar on the whole to these indices for cultures grown in the plasm clot without the substrate.
研究了游离和结合状态的不同剂量胰蛋白酶对聚氨酯基质上大鼠皮下组织成纤维细胞生长的影响。组织培养方法为评估聚合物同种异体植入物的生物不相容程度提供了重要标准，尤其是在将生物活性物质固定在其上时。已证实吸附在聚氨酯基质上并逐渐扩散到培养基中的胰蛋白酶会抑制细胞生长。在培养初期，聚合物基质成分中化学结合的胰蛋白酶会长期刺激成纤维细胞成分的分裂，并进一步加速其退化。结果表明，在不含胰蛋白酶的聚合物基质上，成纤维细胞成分的生长特征和动态总体上与在无基质的血浆凝块中培养的这些指标相似。
