Scheid A, Choppin P W
J Virol. 1973 Feb;11(2):263-71. doi: 10.1128/JVI.11.2.263-271.1973.
A procedure has been developed for the isolation of Newcastle disease virus (NDV) envelope proteins. The two surface glycoproteins and the non-glycosylated membrane protein were solubilized with 2% Triton X-100 and 1 m KCl. Removal of the KCl by dialysis yielded by precipitation a pure preparation of the non-glycosylated membrane protein, which is insoluble in solutions of low ionic strength. The soluble fraction consisting of the two glycoproteins possessed full neuraminidase and hemagglutinating activities. The two glycoproteins could be separated by rate zonal sedimentation in a sucrose gradient containing 1% Triton X-100 and 1 m KCl. Under these conditions, the sedimentation coefficient of the larger glycoprotein, virus protein 1, was 9.3s, and that of the smaller, virus protein 2, was 6.1s. Both hemagglutinating and neuraminidase activities were associated with virus protein 1; virus protein 2 had neither activity. The results suggest that both activities reside on a single NDV glycoprotein. Similar results were obtained previously with another paramyxovirus, simian virus 5. These findings suggest that the association of hemagglutinating and neuraminidase activities with one glycoprotein is a general property of the paramyxovirus group.
已开发出一种分离新城疫病毒（NDV）包膜蛋白的方法。用2% Triton X - 100和1 m KCl溶解两种表面糖蛋白和非糖基化膜蛋白。通过透析去除KCl，经沉淀得到非糖基化膜蛋白的纯制剂，该蛋白不溶于低离子强度溶液。由两种糖蛋白组成的可溶部分具有完整的神经氨酸酶和血凝活性。两种糖蛋白可通过在含有1% Triton X - 100和1 m KCl的蔗糖梯度中进行速率区带沉降分离。在这些条件下，较大糖蛋白病毒蛋白1的沉降系数为9.3s，较小的病毒蛋白2的沉降系数为6.1s。血凝和神经氨酸酶活性均与病毒蛋白1相关；病毒蛋白2没有活性。结果表明这两种活性存在于单一的NDV糖蛋白上。先前用另一种副粘病毒猴病毒5也得到了类似结果。这些发现表明血凝和神经氨酸酶活性与一种糖蛋白的关联是副粘病毒组的普遍特性。
