放线菌素D在正常细胞和病毒转化细胞中的差异作用。
The differential effect of actinomycin D in normal and virus-transformed cells.
作者信息
Williams J G, Macpherson I A
出版信息
J Cell Biol. 1973 Apr;57(1):148-58. doi: 10.1083/jcb.57.1.148.
Abstract
Actinomycin D (AMD) at concentrations up to 0,25 microg/ml shows a differential effect on cell RNA synthesis and on the replication of an influenza virus in normal and virally transformed cells, both functions being more resistant to AMD in the transformed cell. A possible explanation for these differences in AMD sensitivity is provided by the observation that isotopically labeled AMD is maintained at a lower concentration in transformed BHK 21/13 (BHK) cells. There is evidence that the decreased sensitivity of the transformed cells to AMD is a result of maintenance of a lower internal concentration of the drug, since a correlation exists for a number of polyoma virus-transformed clones between sensitivity to and uptake of AMD.
摘要
浓度高达0.25微克/毫升的放线菌素D（AMD）对正常细胞和病毒转化细胞中的细胞RNA合成及流感病毒复制表现出不同的作用，在转化细胞中这两种功能对AMD的抗性更强。观察发现，同位素标记的AMD在转化的BHK 21/13（BHK）细胞中维持在较低浓度，这为AMD敏感性的这些差异提供了一种可能的解释。有证据表明，转化细胞对AMD敏感性降低是药物内部浓度较低的结果，因为在许多多瘤病毒转化的克隆中，对AMD的敏感性与摄取之间存在相关性。
相似文献
1
The differential effect of actinomycin D in normal and virus-transformed cells.放线菌素D在正常细胞和病毒转化细胞中的差异作用。
J Cell Biol. 1973 Apr;57(1):148-58. doi: 10.1083/jcb.57.1.148.
2
The uptake of actinomycin D by normal and virus transformed BHK 21 hamster cells.放线菌素D在正常及病毒转化的BHK 21仓鼠细胞中的摄取情况。
Exp Cell Res. 1975 Mar 15;91(2):237-46. doi: 10.1016/0014-4827(75)90100-7.
3
Studies on the mechanism of actinomycin D resistance of an SV40-transformed hamster cell line.
Exp Cell Res. 1974 Nov;89(1):89-94. doi: 10.1016/0014-4827(74)90190-6.
4
On the differential cytotoxicity of actinomycin D.论放线菌素D的差异细胞毒性。
J Cell Biol. 1971 Sep;50(3):746-61. doi: 10.1083/jcb.50.3.746.
5
Effect of ultraviolet irradiation and actinomycin D on polyoma virus replication in mouse embryo cell cultures.紫外线照射和放线菌素D对小鼠胚胎细胞培养中多瘤病毒复制的影响。
J Virol. 1969 Jun;3(6):570-7. doi: 10.1128/JVI.3.6.570-577.1969.
6
Failure of actinomycin D to inhibit the induction of SV 40 viral antigen in BSC 1 transformed cells.
Arch Gesamte Virusforsch. 1973;41(1):127-31. doi: 10.1007/BF01249937.
7
37RC cells rapidly recover their RNA synthesis after inhibition with high doses of actinomycin D.37RC细胞在用高剂量放线菌素D抑制后能迅速恢复其RNA合成。
J Cell Biol. 1972 Jan;52(1):171-4. doi: 10.1083/jcb.52.1.171.
8
Inhibition of measles virus replication and RNA synthesis by actinomycin D.放线菌素D对麻疹病毒复制和RNA合成的抑制作用。
Biochem Biophys Res Commun. 1972 Aug 7;48(3):657-61. doi: 10.1016/0006-291x(72)90398-1.
9
10
Isolation and preliminary characterization of the RNA-containing R-type, virus-like particle of BHK-21 cells.BHK - 21细胞中含RNA的R型病毒样颗粒的分离与初步鉴定
J Virol. 1973 Nov;12(5):1164-72. doi: 10.1128/JVI.12.5.1164-1172.1973.
引用本文的文献
1
Tumor angiogenesis factor. Speculations on an approach to cancer chemotherapy.肿瘤血管生成因子。关于癌症化疗方法的推测。
Yale J Biol Med. 1974;47(2):71-81.
2
Study on the reinitiation of transcription in 37 RC cells after actinomycin D inhibition. Spectrum of major RNA species resynthesized after maximal suppression by the drug.放线菌素D抑制后37 RC细胞中转录再起始的研究。药物最大抑制后重新合成的主要RNA种类谱。
J Cell Biol. 1975 Dec;67(3):538-50. doi: 10.1083/jcb.67.3.538.
3
Resistance of African green monkey kidney cell lines to actinomycin D: drug uptake in 37 RC cells after persistent inhibition of transcription.非洲绿猴肾细胞系对放线菌素D的抗性：转录持续抑制后37 RC细胞中的药物摄取
Antimicrob Agents Chemother. 1979 Feb;15(2):300-12. doi: 10.1128/AAC.15.2.300.
本文引用的文献
1
Polyoma transformation of hamster cell clones--an investigation of genetic factors affecting cell competence.仓鼠细胞克隆的多瘤病毒转化——对影响细胞感受态的遗传因素的研究
Virology. 1962 Feb;16:147-51. doi: 10.1016/0042-6822(62)90290-8.
2
BIOCHEMICALLY MARKED VARIANTS OF THE SYRIAN HAMSTER FIBROBLAST CELL LINE BHK21 AND ITS DERIVATIVES.叙利亚仓鼠成纤维细胞系BHK21及其衍生物的生化标记变体。
Exp Cell Res. 1965 Apr;38:106-19. doi: 10.1016/0014-4827(65)90432-5.
3
Basis of actinomycin action. I. DNA binding and inhibition of RNA-polymerase synthetic reactions by actinomycin.放线菌素的作用基础。I. 放线菌素与DNA的结合及对RNA聚合酶合成反应的抑制
Proc Natl Acad Sci U S A. 1962 Dec 15;48(12):2094-101. doi: 10.1073/pnas.48.12.2094.
4
Participation of deoxyribonucleic acid in the multiplication of influenza virus.脱氧核糖核酸在流感病毒增殖中的作用。
Nature. 1962 Jun 23;194:1139-40. doi: 10.1038/1941139a0.
5
Giant-size rapidly labeled nuclear ribonucleic acid and cytoplasmic messenger ribonucleic acid in immature duck erythrocytes.未成熟鸭红细胞中的巨大快速标记核糖核酸和细胞质信使核糖核酸
J Mol Biol. 1966 Sep;20(1):145-82. doi: 10.1016/0022-2836(66)90123-9.
6
Influenza virus-induced ribonucleic acid nucleotidyltransferase and the effect of actinomycin D on its formation.流感病毒诱导的核糖核酸核苷酸转移酶及其放线菌素D对其形成的影响。
Biochemistry. 1966 Jan;5(1):231-5. doi: 10.1021/bi00865a030.
7
Incorporation of triated actinomycin D into drug-sensitive and drug-resistant HeLa cells.
Science. 1966 Mar 25;151(3717):1555-6. doi: 10.1126/science.151.3717.1555.
8
Actinomycin binding of normal and phytohaemagglutinin stimulated lymphocytes.放线菌素与正常及植物血凝素刺激的淋巴细胞的结合
Exp Cell Res. 1969 Apr;55(1):120-2. doi: 10.1016/0014-4827(69)90465-0.
9
Uptake in vivo and in vitro of actinomycin D by mouse leukemias as factors in survival.小鼠白血病对放线菌素D的体内外摄取作为生存因素
Biochem Pharmacol. 1968 Jan;17(1):161-4. doi: 10.1016/0006-2952(68)90170-6.
Zotero 插件