Pathiraja N, Abeyratne A S, Perera B M, Buvanendran V
Vet Rec. 1979 Mar 31;104(13):279-81. doi: 10.1136/vr.104.13.279.
Fifty-seven cycling buffalo cows of the river type were treated with two doses of 0.5 mg cloprostenol intramuscularly given 11 days apart. Each animal was inseminated twice at 72 and 96 hours after the second injection of cloprostenol. The first service conception rate diagnosed by rectal palpation at 90 days was 38.6 per cent. At the time of insemination the cervix was easily penetrable on both days in only 39 (68.4 per cent) of the animals. They were inseminated at or beyond the internal cervical os, while the others were inseminated in the cervical canal. There was a marked difference in conception rate between those receiving deep inseminations (48.7 per cent) and the others (16.7 per cent). In relation to the interval from calving to insemination the conception rates for those which had calved 60 to 90, 90 to 120 and 120 to 150 days earlier were 16.6, 36.4 and 55.5 per cent respectively. The use of cloprostenol treatment and fixed-time insemination is a useful method of overcoming the problem of oestrus detection in buffaloes. Acceptable levels of fertility can be obtained in those animals which have a sufficiently relaxed cervix to permit semen deposition at the internal os, provided the interval from calving to insemination is more than 90 days.
对57头河流型泌乳水牛分两次进行肌肉注射，每次注射0.5毫克氯前列醇，间隔11天。在第二次注射氯前列醇后的72小时和96小时，对每头动物进行两次授精。通过直肠触诊在90天时诊断出的首次输精受胎率为38.6%。在授精时，只有39头（68.4%）动物的子宫颈在这两天都易于穿透。对这些动物在子宫颈内口或其上方进行授精，而其他动物则在子宫颈管内授精。深部授精的动物受胎率（48.7%）与其他动物（16.7%）之间存在显著差异。关于产犊至授精的间隔时间，分别在60至90天、90至120天和120至150天前产犊的动物受胎率分别为16.6%、36.4%和55.5%。使用氯前列醇处理和定时授精是克服水牛发情鉴定问题的一种有用方法。对于那些子宫颈足够松弛以允许精液在子宫颈内口沉积的动物，如果产犊至授精的间隔时间超过90天，可以获得可接受的繁殖力水平。
