Perera M A, Pathiraja N, Kumaratilake W L, Abeyratne A S, Buvanendran V
Vet Rec. 1977;101(26-27):520-1.
Cloprostenol, a prostaglandin analogue, was administered intramuscularly to a total of 35 cycling buffalo cows and heifers in two doses, each of 0.5 mg, given 11 days apart. Out of five cows and 12 heifers subjected to observations after the second injection of cloprostenol (day 0), all except one heifer responded. Signs of oestrus were most marked on days 3 or 4. Eighteen treated heifers were kept with buffalo bulls for four days after the second injection while a control group of nine heifers was kept with bulls for 21 days. The first-service conception rate, diagnosed by rectal palpation at 60 days, was 33 1/3 per cent in both groups. Twelve treated heifers were artificially inseminated at 72 and 96 hours after the second injection of cloprostenol, using fresh semen diluted in egg yolk--citrate extender. The first service conception rate at 60 days was 30 per cent.
氯前列醇，一种前列腺素类似物，以0.5毫克的剂量分两次肌肉注射给35头处于发情周期的水牛母牛和小母牛，两次注射间隔11天。在第二次注射氯前列醇（第0天）后接受观察的5头母牛和12头小母牛中，除了一头小母牛外，其他都有反应。发情迹象在第3天或第4天最为明显。18头接受治疗的小母牛在第二次注射后与水牛公牛饲养4天，而9头小母牛的对照组与公牛饲养21天。通过60天直肠触诊诊断的首次配种受胎率，两组均为33又1/3%。12头接受治疗的小母牛在第二次注射氯前列醇后72小时和96小时使用用蛋黄 - 柠檬酸盐稀释液稀释的新鲜精液进行人工授精。60天的首次配种受胎率为30%。
