Pleten' A P, Uchitel' I A, Mazo V K, Nikulicheva S I, Volkova L I
Vopr Med Khim. 1979 Jul-Aug;25(4):441-4.
Isolation and partial purification of renin from human kidney was carried out as follows: defatting of the homogenized kidney; extraction with methyl cellosolve; chromatography on DEAE cellulose; affinity chromatography using immobilized pepstain. Activity of the preparation obtained was 0.1 GU/mg of protein. The preparation of human renin might be used for measurement of renin activity in blood of patients.
将匀浆后的肾脏脱脂；用甲基溶纤剂提取；在DEAE纤维素上进行色谱分离；使用固定化胃蛋白酶抑制剂进行亲和色谱分离。所获制剂的活性为0.1 GU/毫克蛋白质。人肾素制剂可用于测定患者血液中的肾素活性。
