Suppr超能文献

[从人肾中分离肾素]

[Isolation of renin from human kidneys].

作者信息

Pleten' A P, Uchitel' I A, Mazo V K, Nikulicheva S I, Volkova L I

出版信息

Vopr Med Khim. 1979 Jul-Aug;25(4):441-4.

PMID:473685
Abstract

Isolation and partial purification of renin from human kidney was carried out as follows: defatting of the homogenized kidney; extraction with methyl cellosolve; chromatography on DEAE cellulose; affinity chromatography using immobilized pepstain. Activity of the preparation obtained was 0.1 GU/mg of protein. The preparation of human renin might be used for measurement of renin activity in blood of patients.

摘要

从人肾中分离和部分纯化肾素的操作如下

将匀浆后的肾脏脱脂;用甲基溶纤剂提取;在DEAE纤维素上进行色谱分离;使用固定化胃蛋白酶抑制剂进行亲和色谱分离。所获制剂的活性为0.1 GU/毫克蛋白质。人肾素制剂可用于测定患者血液中的肾素活性。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验