Walsh R, Mason D T, Tonkon M J, Wikman-Coffelt J
Prep Biochem. 1976;6(2-3):177-91. doi: 10.1080/00327487608061611.
An affinity column for the purfication of canine plasma renin was prepared using goat anti-renin (dog kidney) gammaG gloublins. The antiserum was prepared against a purified kidney renin preparation. The anti-renin globulins were coupled to cyanogen bromide activated Sepharose. Using the anti-renin globulin-coupled Sepharose as an immuno-adsorbant, a method was devised allowing purification of plasma renin to a 1,000-fold purity.
使用山羊抗肾素（狗肾）γG球蛋白制备了用于纯化犬血浆肾素的亲和柱。抗血清是针对纯化的肾素制剂制备的。抗肾素球蛋白与溴化氰活化的琼脂糖偶联。以抗肾素球蛋白偶联的琼脂糖作为免疫吸附剂，设计了一种方法，可将血浆肾素纯化至1000倍的纯度。
