喹吖因氮芥与小牛胸腺组蛋白组分的相互作用。
Interaction of quinacrine mustard with calf thymus histone fractions.
作者信息
Selander R K, De la Chapelle A
出版信息
Biochem J. 1973 Apr;131(4):757-64. doi: 10.1042/bj1310757.
Abstract
- Interactions of histone fractions with quinacrine mustard were investigated by fluorimetry and spectrophotometry and the results were interpreted with the aid of thinlayer chromatography. 2. Characteristic differences were found between the various histone fractions of calf thymus. The conditions that favoured histone conformational changes and aggregation, also favoured interaction between histones and the dye; low concentrations of SO(4) (2-) brought about more interaction than did Cl(-); urea, guanidinium and iodide ions were inhibitory to binding. 3. Changes in the physical state of all the quinacrine mustard-protein complexes occurred as a function of ionic strength and pH. The most salt-dependent interaction was found in the arginine-rich histone fraction. 4. The interaction of the calf thymus histone fractions with quinacrine mustard was compared with the interaction of bovine plasma albumin and protamine with quinacrine mustard. 5. The relationship between dye-binding and the aggregation of histone fractions was discussed.
摘要
- 通过荧光法和分光光度法研究了组蛋白组分与喹吖因氮芥的相互作用，并借助薄层色谱法对结果进行了解释。2. 在小牛胸腺的各种组蛋白组分之间发现了特征差异。有利于组蛋白构象变化和聚集的条件，也有利于组蛋白与染料之间的相互作用；低浓度的SO(4) (2-) 比Cl(-) 产生更多的相互作用；尿素、胍和碘离子对结合有抑制作用。3. 所有喹吖因氮芥 - 蛋白质复合物的物理状态变化是离子强度和pH的函数。在富含精氨酸的组蛋白组分中发现了最依赖盐的相互作用。4. 将小牛胸腺组蛋白组分与喹吖因氮芥的相互作用与牛血浆白蛋白和鱼精蛋白与喹吖因氮芥的相互作用进行了比较。5. 讨论了染料结合与组蛋白组分聚集之间的关系。
相似文献
1
Interaction of quinacrine mustard with calf thymus histone fractions.喹吖因氮芥与小牛胸腺组蛋白组分的相互作用。
Biochem J. 1973 Apr;131(4):757-64. doi: 10.1042/bj1310757.
2
Interactions of calf-thymus histone fractions in aqueous solution with 8-anilinonaphthalene-1-sulphonic acid.小牛胸腺组蛋白组分在水溶液中与8-苯胺基萘-1-磺酸的相互作用。
Biochem J. 1966 May;99(2):419-26. doi: 10.1042/bj0990419.
3
4
Interaction of quinacrine mustard with mononucleotides and polynucleotides.喹吖因氮芥与单核苷酸和多核苷酸的相互作用。
Biochem J. 1973 Apr;131(4):749-55. doi: 10.1042/bj1310749.
5
Interaction of quinacrine mustard with whole and partially deproteinized calf thymus deoxynucleoproteins.喹吖因氮芥与完整及部分脱蛋白小牛胸腺脱氧核蛋白的相互作用。
Acta Chem Scand B. 1974;28(8):937-48. doi: 10.3891/acta.chem.scand.28b-0937.
6
Modification of histone binding in calf thymus chromatin by protamine.鱼精蛋白对小牛胸腺染色质中组蛋白结合的修饰作用。
Biochemistry. 1975 Jan 14;14(1):122-7. doi: 10.1021/bi00672a021.
7
Fluorescence of quinacrine mustard conjugated to proteins.与蛋白质结合的喹吖因氮芥的荧光。
Arch Biochem Biophys. 1976 Jan;172(1):39-50. doi: 10.1016/0003-9861(76)90045-x.
8
Interactions between arginine-rich histones and deoxyribonucleic acids. II. Circular dichroism.富含精氨酸的组蛋白与脱氧核糖核酸之间的相互作用。II. 圆二色性
Biochemistry. 1976 May 18;15(10):2034-41. doi: 10.1021/bi00655a002.
9
Conformational changes of histone ARE(F3, III).组蛋白ARE（F3，III）的构象变化
Biochemistry. 1974 Nov 19;13(24):4987-92. doi: 10.1021/bi00721a018.
10
Ultracentrifuge tudies of histone fractions from calf thymus deoxyribonucleoprotein.小牛胸腺脱氧核糖核蛋白组蛋白组分的超速离心研究。
Biochem J. 1969 Sep;114(2):227-35. doi: 10.1042/bj1140227.
引用本文的文献
1
Chromosome regions containing DNAs of known base composition, specifically evidenced by 2,7-di-t-butyl proflavine. Comparison with the Q-banding and relation to dye-DNA interactions.含有已知碱基组成DNA的染色体区域，特别是由2,7 - 二叔丁基原黄素明确证实。与Q带的比较以及与染料 - DNA相互作用的关系。
Chromosoma. 1974;47(3):263-81. doi: 10.1007/BF00328861.
2
Dye-nucleoprotein interactions in Giemsa banding.吉姆萨显带中的染料-核蛋白相互作用
J Cell Biol. 1974 Apr;61(1):248-53. doi: 10.1083/jcb.61.1.248.
本文引用的文献
1
A Selective Staining Method for the Basic Proteins of Cell Nuclei.一种细胞核碱性蛋白质的选择性染色方法。
Proc Natl Acad Sci U S A. 1953 Oct;39(10):991-9. doi: 10.1073/pnas.39.10.991.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
[STUDY OF THE METACHROMASIA OF EOSIN IN THE PRESENCE OF HISTONES].
C R Hebd Seances Acad Sci. 1964 May 20;258:5087-90.
4
The ultraviolet fluorescence of proteins in neutral solution.中性溶液中蛋白质的紫外荧光。
Biochem J. 1960 Aug;76(2):381-8. doi: 10.1042/bj0760381.
5
Cell specificity of beta-histones in the domestic fowl.家鸡β-组蛋白的细胞特异性
Proc R Soc Lond B Biol Sci. 1959 Apr 21;150(940):299-311. doi: 10.1098/rspb.1959.0023.
6
On the molecular weight of calf thymus histone.关于小牛胸腺组蛋白的分子量
Biochim Biophys Acta. 1956 Oct;22(1):205-6. doi: 10.1016/0006-3002(56)90249-9.
7
Histones of genetically active and inactive chromatin.基因活性染色质和非活性染色质的组蛋白。
J Cell Biol. 1967 Dec;35(3):699-708. doi: 10.1083/jcb.35.3.699.
8
Interactions of calf-thymus histone fractions in aqueous solution with 8-anilinonaphthalene-1-sulphonic acid.小牛胸腺组蛋白组分在水溶液中与8-苯胺基萘-1-磺酸的相互作用。
Biochem J. 1966 May;99(2):419-26. doi: 10.1042/bj0990419.
9
A fluorescence assay suitable for histone solutions.
Anal Biochem. 1965 Jun;11(3):443-8. doi: 10.1016/0003-2697(65)90063-1.
10
Studies on histones. 7. Preparative methods for histone fractions from calf thymus.组蛋白研究。7. 从小牛胸腺中制备组蛋白组分的方法。
Biochem J. 1964 Jul;92(1):55-9. doi: 10.1042/bj0920055.
Zotero 插件