喹吖因氮芥与单核苷酸和多核苷酸的相互作用。
Interaction of quinacrine mustard with mononucleotides and polynucleotides.
作者信息
Selander R K
出版信息
Biochem J. 1973 Apr;131(4):749-55. doi: 10.1042/bj1310749.
Abstract
- The interaction between quinacrine mustard and mononucleotides and polynucleotides was investigated by fluorimetry and absorbance spectrophotometry. 2. The fluorescence spectrum of quinacrine mustard is independent of the ionic strength and pH. The dependence of the quinacrine mustard fluorescence intensity on ionic strength, pH and anions is described. 3. The fluorescence intensity of quinacrine mustard was enhanced with the mononucleotide adenylic acid and polynucleotides such as poly(rA), poly(rU) and poly(rA,rU). 4. Quenching of the fluorescence intensity of quinacrine mustard occurred with the mononucleotide guanylic acid and with poly(rG) and poly(rC,rG). 5. The mononucleotide cytidylic acid or poly(rC) showed no effect on the fluorescence intensity of quinacrine mustard. 6. The interaction between the dye and native DNA species was also dependent on the presence of base-specific binding sites in the DNA. The higher the (G+C) content was in the native DNA tested the higher was the quenching effect on the fluorescence intensity of quinacrine mustard. 7. No interaction was found between the dye and methylated DNA. The binding between quinacrine mustard and apurinic DNA was confirmed to be in the phosphate groups of the purines.
摘要
- 通过荧光测定法和吸收分光光度法研究了喹吖因芥子气与单核苷酸和多核苷酸之间的相互作用。2. 喹吖因芥子气的荧光光谱与离子强度和pH无关。描述了喹吖因芥子气荧光强度对离子强度、pH和阴离子的依赖性。3. 喹吖因芥子气的荧光强度随单核苷酸腺苷酸以及多核苷酸如聚（rA）、聚（rU）和聚（rA，rU）而增强。4. 喹吖因芥子气的荧光强度会因单核苷酸鸟苷酸以及聚（rG）和聚（rC，rG）而猝灭。5. 单核苷酸胞苷酸或聚（rC）对喹吖因芥子气的荧光强度没有影响。6. 染料与天然DNA种类之间的相互作用也取决于DNA中碱基特异性结合位点的存在。所测试的天然DNA中（G + C）含量越高，对喹吖因芥子气荧光强度的猝灭作用就越强。7. 未发现染料与甲基化DNA之间存在相互作用。证实喹吖因芥子气与脱嘌呤DNA之间的结合存在于嘌呤的磷酸基团中。
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