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通过臭氧分解从红细胞糖鞘脂释放的寡糖的气液色谱分析。

Gas-liquid chromatography of oligosaccharides released from red cell glycosphingolipids by ozonolysis.

作者信息

Oashi M, Yamakawa T

出版信息

J Lipid Res. 1973 Nov;14(6):698-700.

PMID:4742565
Abstract

A method for the analysis of glycosphingolipids in mammalian erythrocyte membranes is described. It consists of ozonolysis and alkaline treatment of the crude lipid extract to obtain oligosaccharides from glycosphingolipids and then gas-liquid chromatography of trimethylsilyl derivatives of glycitols derived from the oligosaccharides. Typical gas-liquid chromatographic patterns of oligosaccharide components were obtained with various mammalian erythrocytes; these corresponded to the glycosphingolipid compositions. The analysis could be carried out on 10 ml of packed erythrocytes.

摘要

本文描述了一种分析哺乳动物红细胞膜中糖鞘脂的方法。该方法包括对粗脂质提取物进行臭氧分解和碱处理，以从糖鞘脂中获得寡糖，然后对寡糖衍生的糖醇的三甲基硅烷基衍生物进行气液色谱分析。用各种哺乳动物红细胞获得了寡糖成分的典型气液色谱图谱；这些图谱与糖鞘脂组成相对应。该分析可在10毫升压实红细胞上进行。

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