Plummer G, Goodheart C R, Studdert M J
Infect Immun. 1973 Oct;8(4):621-7. doi: 10.1128/iai.8.4.621-627.1973.
Equine herpesviruses with a deoxyribonucleic acid density of 1.716 to 1.717 g/cm(3) were compared with one another by the plaque-reduction test and by the rate of development of cytopathic effect as indicated by plaque size in rabbit kidney cultures. Of the 19 isolates studied, the 9 which had already been tentatively labeled equine abortion viruses were serologically similar to one another; each of them grew more quickly than did any of the other 10 isolates although the mean plaque sizes formed a series of gradations with no clear hiatus which would permit the unequivocal delineation of the abortion viruses from the slowly growing strains. The 10 slowly growing isolates showed antigenic heterogeneity even though complement was present; the neutralizing capacity of an antiserum against the heterologous strains was, in most instances, markedly less than against the homologous strains, the range of the 50% endpoints being much greater than that observed among the equine abortion viruses, or among isolates of herpes simplex type 1. There was no cross neutralization between the equine abortion viruses and any of the 10 slowly growing isolates. An extra band of deoxyribonucleic acid, at 1.723 to 1.725 g/cm(3), was present in two of the slowly growing strains when originally grown in rabbit cells, but was no longer present after passage in cat cells. This band occupied the same position as one reported in the hamster-passaged strain of equine abortion virus, and had a density similar to that of the equine genital herpesvirus. Although the taxonomic demarcation of the equine abortion viruses and the slowly growing herpesviruses from one another is still open to question, they can be conveniently labeled equine herpesviruses 1 and 2, respectively; the genital virus would be termed equine herpesvirus 3.
通过蚀斑减少试验以及兔肾培养物中蚀斑大小所表明的细胞病变效应发展速率，对脱氧核糖核酸密度为1.716至1.717 g/cm³的马疱疹病毒进行了相互比较。在所研究的19个分离株中，9个已初步标记为马流产病毒的分离株在血清学上彼此相似；它们中的每一个生长速度都比其他10个分离株快，尽管平均蚀斑大小形成了一系列渐变，没有明显的间断，无法明确区分流产病毒和生长缓慢的毒株。10个生长缓慢的分离株即使存在补体也表现出抗原异质性；在大多数情况下，抗血清对异源毒株的中和能力明显低于对同源毒株的中和能力，50%终点的范围比在马流产病毒或单纯疱疹病毒1型分离株中观察到的范围大得多。马流产病毒与10个生长缓慢的分离株中的任何一个之间都没有交叉中和作用。最初在兔细胞中生长时，两个生长缓慢的毒株中存在一条额外的脱氧核糖核酸带，密度为1.723至1.725 g/cm³，但在猫细胞传代后不再存在。这条带占据的位置与马流产病毒仓鼠传代株中报道的一条带相同，密度与马生殖器疱疹病毒的密度相似。尽管马流产病毒和生长缓慢的疱疹病毒在分类学上的划分仍有疑问，但它们可以分别方便地标记为马疱疹病毒1型和2型；生殖器病毒将被称为马疱疹病毒3型。
