Michael L H, Lewis R M, Brandon T A, Entman M L
Am J Physiol. 1979 Sep;237(3):H311-7. doi: 10.1152/ajpheart.1979.237.3.H311.
The cardiac lymphatic duct was cannulated in dogs and the exteriorized cannula allowed chronic collection of lymph during the awake state for as long as 3 wk. The surgical methodology and inherent difficulties in the technique are describ:d. Cardiac lymph flow ranged from 0.45--5.6 ml/h in the control state in 14 dogs. An occluding device and flow probe were placed on the circumflex coronary artery (CFX); ultrasonic segment length crystals were placed in the left ventricular free wall in 4 dogs. Occlusion of the CFX in these conscious dogs caused lymph flow to fall as great as 46% below control during the 1st half-hour. Reperfusion of the occluded vessel caused an increase in lymph flow as great as 67% above control. The effect on cardiac lymph flow was demonstrated for a few select drugs that have known effects on the cardiovascular system. Cardiac lymph flow was altered from control as follows: isoproterenol, 42 +/- 11% increase; RO 2-2985, 118 +/- 8% increase; verapamil, 101 +/- 10% increase; propranolol caused no significant change. The conscious dog with cardiac lymph vessel cannulated should provide a model to further study the complexities of cardiac metabolism and physiology without interference of anesthesia and surgical stress.
在犬身上对心脏淋巴管进行插管，并将外置插管用于在清醒状态下长期收集淋巴液，长达3周。描述了该手术方法及该技术中固有的困难。14只犬在对照状态下心脏淋巴液流量为0.45 - 5.6毫升/小时。在左旋冠状动脉（CFX）上放置一个闭塞装置和流量探头；在4只犬的左心室游离壁上放置超声节段长度晶体。在这些清醒犬中，CFX闭塞在最初半小时内使淋巴液流量比对照降低多达46%。闭塞血管再灌注使淋巴液流量比对照增加多达67%。对几种已知对心血管系统有作用的特定药物，展示了其对心脏淋巴液流量的影响。心脏淋巴液流量与对照相比变化如下：异丙肾上腺素，增加42±11%；RO 2 - 2985，增加118±8%；维拉帕米，增加101±10%；普萘洛尔无显著变化。插管了心脏淋巴管的清醒犬应能提供一个模型，用于在无麻醉和手术应激干扰的情况下进一步研究心脏代谢和生理学的复杂性。
