Michael L H, Hunt J R, Weilbaecher D, Perryman M B, Roberts R, Lewis R M, Entman M L
Am J Physiol. 1985 Mar;248(3 Pt 2):H350-9. doi: 10.1152/ajpheart.1985.248.3.H350.
Cardiac lymph, collected from conscious dogs, was monitored for glycogen phosphorylase and creatine kinase (CK) enzymatic activity during control state, circumflex coronary artery (CFX) occlusion, and reperfusion. CFX occlusions, lasting for intervals as short as 10 min, initiated a release of phosphorylase and CK into the cardiac lymph, which was immediately observed during reperfusion of the ischemic tissue. Blood plasma levels did not appear for several hours. In the absence of reperfusion, the appearance of enzymes in cardiac lymph was delayed and peaked later. Glycogen phosphorylase and CK entered the lymph in greater quantities with reperfusion as the length of occlusion was increased. Histological examination of multiple sections of the reperfused hearts showed infarcts in hearts where CFX occlusions lasted 20 min or longer; occlusions of 10-15 min showed evidence of cell injury and death in two hearts and no definable infarct in the majority. Ischemic intervals of short duration release functionally active glycogen phosphorylase and CK, which reflect changes in myocardial cell egress of macromolecules and/or cell death.
从清醒犬收集心脏淋巴液，在对照状态、左旋冠状动脉（CFX）闭塞及再灌注期间监测其糖原磷酸化酶和肌酸激酶（CK）的酶活性。CFX闭塞持续时间短至10分钟，引发磷酸化酶和CK释放至心脏淋巴液中，在缺血组织再灌注期间立即观察到这种情况。血浆水平在数小时内未出现。在无再灌注的情况下，心脏淋巴液中酶的出现延迟且峰值出现较晚。随着闭塞时间延长，再灌注时糖原磷酸化酶和CK进入淋巴液的量增加。对再灌注心脏的多个切片进行组织学检查发现，CFX闭塞持续20分钟或更长时间的心脏出现梗死；10 - 15分钟的闭塞在两颗心脏中显示出细胞损伤和死亡的证据，大多数心脏无明确梗死。短时间的缺血间隔释放功能活跃的糖原磷酸化酶和CK，这反映了心肌细胞大分子流出和/或细胞死亡的变化。
