将标记前体掺入乳酸脱氢酶病毒的RNA和蛋白质中。
Incorporation of labeled precursors into the RNA and proteins of lactic dehydrogenase virus.
作者信息
Niwa A, Yamazaki S, Bader J, Notkins A L
出版信息
J Virol. 1973 Aug;12(2):401-4. doi: 10.1128/JVI.12.2.401-404.1973.
Abstract
Lactic dehydrogenase virus was grown in primary mouse embryo cells and labeled with (3)H-uridine and (3)H-amino acids. Concentrated and purified virus was banded by isopycnic centrifugation in sucrose gradients, and infectivity and radioactivity were found to correspond at a density of 1.17 g/cm(3). The extracted viral RNA was resolved by electrophoresis in polyacrylamide-agarose mixed gels, and the mol wt was estimated to be 6.0 x 10(6).
摘要
乳酸脱氢酶病毒在原代小鼠胚胎细胞中培养，并用³H-尿苷和³H-氨基酸进行标记。浓缩和纯化后的病毒通过在蔗糖梯度中进行等密度离心进行分带，发现在密度为1.17 g/cm³时，感染性和放射性相对应。提取的病毒RNA在聚丙烯酰胺-琼脂糖混合凝胶中通过电泳进行分离，其分子量估计为6.0×10⁶。
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