乳酸脱氢酶升高病毒及其核糖核酸的物理性质
Physical properties of lactic dehydrogenase-elevating virus and its ribonucleic acid.
作者信息
Darnell M B, Plagemann P G
出版信息
J Virol. 1972 Nov;10(5):1082-5. doi: 10.1128/JVI.10.5.1082-1085.1972.
Abstract
Infectious lactic dehydrogenase-elevating virus propagated in primary cultures of mouse peritoneal macrophages in the presence of (3)H-uridine and isolated by isopycnic centrifugation was found to have a density of 1.12 g/cm(3). Ribonucleic acid extracted from the virus by treatment with sodium dodecyl sulfate was single stranded with a sedimentation coefficient of approximately 48S.
摘要
在存在³H-尿苷的情况下，在小鼠腹膜巨噬细胞原代培养物中繁殖并通过等密度离心分离的传染性乳酸脱氢酶升高病毒，其密度为1.12 g/cm³。用十二烷基硫酸钠处理从该病毒中提取的核糖核酸为单链，沉降系数约为48S。
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