Philp J R, Johnson J E, Spencer J C
Infect Immun. 1973 Nov;8(5):781-6. doi: 10.1128/iai.8.5.781-786.1973.
When lymphocyte-macrophage suspensions from sensitized animals are preincubated with specific antigen for 24 or more h, the following results are observed. (i) In a standard capillary macrophage migration test, there is complete inhibition of migration. (ii) When the preincubated cell suspension is mixed in varying proportions with a similar suspension from nonsensitized animals and a macrophage migration test is performed, there is no linear relationship between the degree of inhibition of migration and the proportion of sensitized lymphocytes initially present. Inhibition thus appears to be an "all-or-none" effect. (iii) In spite of the second observation, increasing periods of preincubation with antigen result in increasing inhibition. (iv) These results suggest the existence of a complex amplifying mechanism operating within the early period of exposure to antigen. (v) To test the possibility that cell proliferation contributes to this amplification, cells from sensitized guinea pigs were irradiated with a dose of 1,000 rads prior to preincubation with antigen. Despite this dose, which virtually abolishes cell division in other systems, no diminution whatever in the amplification of inhibition was observed. These results suggest the existence of an early phase of increased production of migratory inhibition factor that is not dependent on cell division but that may be related to "recruitment" of nonsensitized lymphocytes.
当将来自致敏动物的淋巴细胞 - 巨噬细胞悬液与特异性抗原预孵育24小时或更长时间时，可观察到以下结果。（i）在标准的毛细管巨噬细胞迁移试验中，迁移完全受到抑制。（ii）当将预孵育的细胞悬液与来自未致敏动物的类似悬液按不同比例混合并进行巨噬细胞迁移试验时，迁移抑制程度与最初存在的致敏淋巴细胞比例之间不存在线性关系。因此，抑制似乎是一种“全或无”效应。（iii）尽管有第二项观察结果，但抗原预孵育时间的延长会导致抑制作用增强。（iv）这些结果表明在接触抗原的早期存在一种复杂的放大机制。（v）为了测试细胞增殖是否有助于这种放大作用，在与抗原预孵育之前，用1000拉德的剂量照射致敏豚鼠的细胞。尽管该剂量在其他系统中几乎消除了细胞分裂，但未观察到抑制作用放大有任何减弱。这些结果表明存在迁移抑制因子产生增加的早期阶段，其不依赖于细胞分裂，但可能与未致敏淋巴细胞的“募集”有关。
