利用N-甲基-N'-硝基-N-亚硝基胍从多杀性巴氏杆菌和溶血巴氏杆菌中获得链霉素依赖型突变体的改良方法。
Improved method for obtaining streptomycin-dependent mutants from Pasteurella multocida and Pasteurella haemolytica, using N-methyl-N'-nitro-N-nitrosoguanidine.
作者信息
Chengappa M M, Carter G R
出版信息
Am J Vet Res. 1979 Mar;40(3):449-50.
PMID:475104
Abstract
N-Methyl-N'-nitro-N-nitrosoguanidine was used in a modified and improved procedure to obtain streptomycin-dependent mutants of type A Pasteurella multocida and type 1 Pasteurella haemolytica. The relative virulence of these mutants was determined by mouse inoculation with and without streptomycin.
摘要
N-甲基-N'-硝基-N-亚硝基胍用于一种改良和改进的程序，以获得A型多杀巴斯德菌和1型溶血巴斯德菌的链霉素依赖突变体。通过在有和没有链霉素的情况下对小鼠进行接种来测定这些突变体的相对毒力。
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