利用气相色谱法检测细菌中的鸟氨酸脱羧酶和赖氨酸脱羧酶活性。
Use of gas chromatography for detecting ornithine and lysine decarboxylase activity in bacteria.
作者信息
Lambert M A, Moss C W
出版信息
Appl Microbiol. 1973 Oct;26(4):517-20. doi: 10.1128/am.26.4.517-520.1973.
A gas-liquid chromatography (GLC) procedure for the detection of L-ornithine and L-lysine decarboxylase (EC 4.1.1.17 and EC 4.1.1.18, respectively) activities of bacteria was developed and evaluated against Møller's method, a conventional biochemical test. Cultures were incubated for 2 to 4 h in a simple growth medium and tested by GLC for putrescine and cadaverine, the direct decarboxylation products of ornithine and lysine, respectively. Results obtained with various Enterobacteriaceae, pseudomonads, and vibrios showed that the GLC procedure was superior to the conventional test; clear, well-defined results were obtained within 3 to 5 h, even with cultures which gave weak, delayed, or variable reactions by Møller's method. This GLC procedure for the determination of decarboxylase reactions would be useful in microbiological laboratories for culture identification and for various other enzymatic studies.
开发了一种用于检测细菌L-鸟氨酸和L-赖氨酸脱羧酶（分别为EC 4.1.1.17和EC 4.1.1.18）活性的气液色谱（GLC）方法，并与传统生化试验Møller法进行了评估。将培养物在简单生长培养基中孵育2至4小时，然后通过GLC检测腐胺和尸胺，它们分别是鸟氨酸和赖氨酸的直接脱羧产物。用各种肠杆菌科细菌、假单胞菌和弧菌获得的结果表明，GLC方法优于传统试验；即使是用Møller法产生微弱、延迟或可变反应的培养物，也能在3至5小时内获得清晰、明确的结果。这种用于测定脱羧酶反应的GLC方法在微生物实验室中可用于培养物鉴定和各种其他酶学研究。
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