Oberhofer T R, Rowen J W, Higbee J W, Johns R W
J Clin Microbiol. 1976 Feb;3(2):137-42. doi: 10.1128/jcm.3.2.137-142.1976.
A rapid medium for the detection of lysine and ornithine decarboxylase and arginine dihydrolase activity of 439 strains of gram-negative, nonfermenting bacteria was evaluated and compared with Moeller decarboxylase medium. Results were obtained in 4 to 24 h using the rapid medium, whereas Moeller medium often required extended (3 to 7 days) incubation. There was 100% agreement in the lysine tests with both media and almost 100% agreement in the ornithine tests. There was 91% agreement in the arginine tests, with the significance of discrepant results discussed. The sensitivity, specificity, and quick results obtained by the rapid test make it a suitable substitute for Moeller medium for the identication of gram-negative, nonfermenting bacteria.
对一种用于检测439株革兰氏阴性非发酵菌赖氨酸脱羧酶、鸟氨酸脱羧酶和精氨酸双水解酶活性的快速培养基进行了评估,并与默勒脱羧酶培养基进行了比较。使用快速培养基在4至24小时内获得结果,而默勒培养基通常需要延长(3至7天)培养时间。两种培养基在赖氨酸检测中的一致性为100%,在鸟氨酸检测中的一致性几乎为100%。在精氨酸检测中的一致性为91%,并讨论了结果不一致的意义。快速检测所获得的敏感性、特异性和快速结果使其成为用于鉴定革兰氏阴性非发酵菌的默勒培养基的合适替代品。