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Lysosomes in rat thoracic duct lymphocytes fractionated by zonal centrifugation.通过区带离心法分离的大鼠胸导管淋巴细胞中的溶酶体。
J Cell Biol. 1973 Oct;59(1):177-84. doi: 10.1083/jcb.59.1.177.
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Lysosomes in rat thoracic duct lymphocytes.大鼠胸导管淋巴细胞中的溶酶体
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本文引用的文献

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Plaque production by the polyoma virus.多瘤病毒的噬菌斑产生
Virology. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1.
2
Plaque formation and isolation of pure lines with poliomyelitis viruses.脊髓灰质炎病毒的噬斑形成及纯系分离
J Exp Med. 1954 Feb;99(2):167-82. doi: 10.1084/jem.99.2.167.
3
Lysosomes in lymphoid tissue. III. Influence of various treatments of the animals on the distribution of acid hydrolases.淋巴组织中的溶酶体。III. 动物的各种处理对酸性水解酶分布的影响。
J Cell Biol. 1967 Feb;32(2):349-64. doi: 10.1083/jcb.32.2.349.
4
Lysosomes in lymphoid tissue. II. Intracellular distribution of acid hydrolases.淋巴组织中的溶酶体。II. 酸性水解酶的细胞内分布。
J Cell Biol. 1967 Feb;32(2):339-48. doi: 10.1083/jcb.32.2.339.
5
Analytical techniques for cell fractions. V. Characteristics of the B-XIV and B-XV zonal centrifuge rotors.细胞组分的分析技术。V. B-XIV和B-XV区带离心机转子的特性
Anal Biochem. 1967 Nov;21(2):235-52. doi: 10.1016/0003-2697(67)90185-6.
6
Analytical techniques for cell fractions. VII. A simple gradient-forming apparatus.细胞组分的分析技术。VII. 一种简单的梯度形成装置。
Anal Biochem. 1967 Nov;21(2):259-65. doi: 10.1016/0003-2697(67)90187-x.
7
Identification of marrow-derived and thymus-derived small lymphocytes in the lymphoid tissue and thoracic duct lymph of normal rats.正常大鼠淋巴组织和胸导管淋巴中骨髓源性和胸腺源性小淋巴细胞的鉴定。
J Exp Med. 1972 Feb 1;135(2):200-19. doi: 10.1084/jem.135.2.200.
8
The life-span and recirculation of marrow-derived small lymphocytes from the rat thoracic duct.大鼠胸导管中骨髓来源的小淋巴细胞的寿命和再循环
J Exp Med. 1972 Feb 1;135(2):185-99. doi: 10.1084/jem.135.2.185.
9
Lysosomes in rat thoracic duct lymphocytes.大鼠胸导管淋巴细胞中的溶酶体
J Exp Med. 1972 Dec 1;136(6):1394-403. doi: 10.1084/jem.136.6.1394.

通过区带离心法分离的大鼠胸导管淋巴细胞中的溶酶体。

Lysosomes in rat thoracic duct lymphocytes fractionated by zonal centrifugation.

作者信息

Bowers W E

出版信息

J Cell Biol. 1973 Oct;59(1):177-84. doi: 10.1083/jcb.59.1.177.

DOI:10.1083/jcb.59.1.177
PMID:4752404
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2110916/
Abstract

A method of zonal centrifugation was developed which separates rat thoracic duct lymphocytes (TDL) mainly according to size. The validity of the fractionation method was supported by light microscope observations, Coulter Counter sizing, and in vivo and in vitro labeling of lymphocytes. The distributions of lysosomal acid hydrolases in TDL fractionated by zonal centrifugation are similar to the distribution obtained for the cells. This result indicates that the large lymphocyte is not the sole bearer of either lysosomes or the large amount of soluble cathepsin D found in homogenates of TDL. Both reside mainly in small lymphocytes. This point was clearly established by fractionating homogenates of purified small lymphocytes by means of differential centrifugation and isopycnic density gradient centrifugation.

摘要

开发了一种区带离心方法，该方法主要根据大小分离大鼠胸导管淋巴细胞（TDL）。通过光学显微镜观察、库尔特计数器测量大小以及淋巴细胞的体内和体外标记，支持了分级分离方法的有效性。通过区带离心分级分离的TDL中溶酶体酸性水解酶的分布与细胞的分布相似。该结果表明，大淋巴细胞不是TDL匀浆中发现的溶酶体或大量可溶性组织蛋白酶D的唯一载体。两者主要存在于小淋巴细胞中。通过差速离心和等密度密度梯度离心对纯化的小淋巴细胞匀浆进行分级分离，明确证实了这一点。