Smith E L, Bowdler A J, Bull R W, McKibbin J M
Immunology. 1973 Oct;25(4):621-9.
A number of fucose-containing glycolipids (fuco-lipids), which are similar in composition to those of human normal and malignant gastrointestinal tissue, have been isolated from whole small intestines of individual dogs. Dogs from which these fuco-lipids were isolated fell into two types according to the qualitative sugar composition of their fuco-lipids. Glycolipids from type I dogs contained glucose, galactose, glucosamine, galactosamine and fucose, while those from type II dogs contained the same sugars but lacked galactosamine. Fucolipids isolated from type I and II dogs were tested for both canine blood group and human A, B, H and Le and Le blood group activity. At the concentrations tested, only human blood group A activity was found in significant amounts, and only in those fuco-lipids which contained galactosamine (type I dogs). Of the fuco-lipids with human blood group A activity, some had activity comparable to that of glycoprotein blood group substances, while others had lower, but significant, activity. These latter fuco-lipids also had marked chromatographic differences, indicating that they are of several different structural types, a finding similar to the A active glycolipids of human red cell stroma. None of the isolated intestinal fuco-lipids had canine blood group activity. A fuco-lipid with Le activity was also isolated in relatively large amounts from a normal human whole small intestine.
从个别犬的整个小肠中分离出了一些含岩藻糖的糖脂（岩藻糖脂），其组成与人类正常和恶性胃肠道组织的糖脂相似。根据其岩藻糖脂的定性糖组成，分离出这些岩藻糖脂的犬分为两种类型。I型犬的糖脂含有葡萄糖、半乳糖、葡萄糖胺、半乳糖胺和岩藻糖，而II型犬的糖脂含有相同的糖类，但不含半乳糖胺。对从I型和II型犬分离出的岩藻糖脂进行了犬血型以及人类A、B、H和Lea及Leb血型活性测试。在所测试的浓度下，仅在含有半乳糖胺的岩藻糖脂（I型犬）中发现了大量的人类A型血型活性。在具有人类A型血型活性的岩藻糖脂中，有些的活性与糖蛋白血型物质相当，而另一些的活性较低，但很显著。这些后者的岩藻糖脂在色谱上也有明显差异，表明它们属于几种不同的结构类型，这一发现与人类红细胞基质的A活性糖脂相似。分离出的肠道岩藻糖脂均无犬血型活性。还从正常人类整个小肠中相对大量地分离出了一种具有Lea活性的岩藻糖脂。
