Lefford M J, McGregor D D, Mackaness G B
Immunology. 1973 Oct;25(4):703-15.
Following immunization of rats with BCG there was added to thoracic duct lymph a population of sensitized lymphocytes which conferred adoptive immunity to tuberculosis upon syngeneic recipients. At an early stage of immunization, 5 days after BCG inoculation, the sensitized cells were large and medium lymphocytes as evidenced by their sensitivity to vinblastine. On day 8, the sensitized cell population was partially resistant to vinblastine and by day 28 it was totally resistant. The vinblastine resistant cells have the functional properties of small lymphocytes, since they recirculate freely from the blood to lymph, and can confer long lasting immunity upon recipient rats. The data imply that protective immunity against the tubercle bacillus is transferred by a family of newly-formed lymphocytes, the most immature of which are immunoblasts or large lymphocytes. As the response matures, sensitized small lymphocytes are delivered to the circulation and comprise an increasing proportion of the protective cell population.
用卡介苗免疫大鼠后，向胸导管淋巴液中加入一群致敏淋巴细胞，这些细胞可使同基因受体获得对结核病的过继免疫。在免疫早期，卡介苗接种后5天，致敏细胞为大淋巴细胞和中淋巴细胞，这可通过它们对长春碱的敏感性得到证明。在第8天，致敏细胞群体对长春碱部分耐药，到第28天则完全耐药。长春碱耐药细胞具有小淋巴细胞的功能特性，因为它们能从血液自由循环到淋巴液，并能赋予受体大鼠持久免疫力。数据表明，针对结核杆菌的保护性免疫是由一群新形成的淋巴细胞传递的，其中最不成熟的是免疫母细胞或大淋巴细胞。随着反应的成熟，致敏小淋巴细胞进入循环，在保护性细胞群体中所占比例越来越大。
