Lefford M J
Immunology. 1980 Nov;41(3):643-51.
Rats were immunized with living BCG and acute peritoneal exudates were induced on the ninth day of infection. The peritoneal exudate cells (PEC), that confer adoptive anti-tuberculosis immunity and tuberculin delayed-type hypersensitivity (DTH), were subject to velocity sedimentation analysis. It was found that the ability to confer immunity of DTH was limited to a population of cells that sedimented at a rate of 3-4 mm/h. This sedimentation rate corresponds to that of small lymphocytes. No significant immunological activity was detected in large lymphocytes that incorporate [3H]-thymidine in vitro, regardless of whether the exudates were obtained 14 to 24 h after induction of peritoneal inflammation. The failure of large lymphocytes to confer immunity and DTH was not due to adherent cells with suppressor activity, because removal of adherent cells failed to amplify the transfer of immunological activity by non-adherent cells. The persistence of the ability to express immunity and DTH in adoptively immunized rats was studied. There was no decay of adoptive immunity during a 4 week period following cell transfer, but there was a rapid reduction in the expression of DTH.
用活卡介苗免疫大鼠，并在感染的第9天诱导急性腹膜渗出液。对赋予过继性抗结核免疫力和结核菌素迟发型超敏反应（DTH）的腹膜渗出细胞（PEC）进行速度沉降分析。结果发现，赋予DTH免疫力的能力仅限于以3 - 4毫米/小时的速度沉降的细胞群体。这个沉降速度与小淋巴细胞的沉降速度相当。无论腹膜炎症诱导后14至24小时是否获得渗出液，体外掺入[3H] - 胸腺嘧啶核苷的大淋巴细胞均未检测到明显的免疫活性。大淋巴细胞未能赋予免疫力和DTH并非由于具有抑制活性的黏附细胞，因为去除黏附细胞并不能增强非黏附细胞的免疫活性传递。研究了过继免疫大鼠中表达免疫力和DTH能力的持久性。细胞转移后4周内过继免疫力没有衰退，但DTH的表达迅速降低。
