Kamikawa Y, Shimo Y
Arch Int Pharmacodyn Ther. 1979 Apr;238(2):220-32.
Transmural stimulation of the isolated muscularis mucosae from guinea-pig esophagus induced contraction, which was completely inhibited by the pretreatment with atropine (o.1 microM) or tetrodotoxin (0.1 microM). In the presence of atropine, muscularis mucosae which contracted with histamine 2 microM slightly relaxed by transmural stimulation in 8 out of 20 preparations. The relaxation was inhibited by an addition of guanethidine (10 microM) or propranolol (2 microM). The vagal stimulation or an addition of nicotine also induced contractions of muscularis mucosae which were blocked by hexamethonium (20-50 microM), tetrodotoxin (0.1 microM) or atropine (0.1 microM). Using the formaldehydeinduced fluorescence technique, a few catecholamine-containing nerve fibres were observed in muscularis mucosae or in the wall of small blood vessels of guinea-pig esophagus. From these results, it is suggested that muscularis mucosae in guinea-pig esophagus is innervated mostly with excitatory cholinergic nerves, and sparsely with inhibitory adrenergic nerves, but not with non-adrenergic and non-cholinergic nerves.
对豚鼠食管分离的黏膜肌层进行透壁刺激可诱发收缩，该收缩可被用阿托品（0.1微摩尔）或河豚毒素（0.1微摩尔）预处理完全抑制。在阿托品存在的情况下，20份标本中有8份，用2微摩尔组胺收缩的黏膜肌层经透壁刺激后稍有松弛。加入胍乙啶（10微摩尔）或普萘洛尔（2微摩尔）可抑制这种松弛。迷走神经刺激或加入尼古丁也可诱发黏膜肌层收缩，这可被六甲铵（20 - 50微摩尔）、河豚毒素（0.1微摩尔）或阿托品（0.1微摩尔）阻断。利用甲醛诱导荧光技术，在豚鼠食管的黏膜肌层或小血管壁中观察到一些含儿茶酚胺的神经纤维。从这些结果提示，豚鼠食管的黏膜肌层主要由兴奋性胆碱能神经支配，少量由抑制性肾上腺素能神经支配，而非由非肾上腺素能和非胆碱能神经支配。
