从BP8小鼠腹水肿瘤中制备无细胞肿瘤抗原的方法研究。
A study of methods for producing cell-free tumour antigen from BP8 mouse ascites tumour.
作者信息
Davies E G, Cater D B
出版信息
Br J Exp Pathol. 1973 Oct;54(5):583-9.
One million lymph node cells (LNC) from C57 B1 mice immunized with BP8 cells plus Freund's complete adjuvant (FCA) protected C3H mice against fatal challenge with BP8 injected i.p. 20 hours later. The LNC of C57 B1 mice immunized with the supernatant from BP8 cells treated with lysolecithin (1·5 μg/10 cells) gave equally good protection. BP8 cells after treatment with lysolecithin equalled untreated BP8 cells in antigenic efficiency. Cell-free preparations made from BP8 cells with 3 molar KCl were of doubtful antigenic efficiency. The LNC from C57 B1 mice immunized with C3H liver and spleen tissue plus FCA gave no protection and LNC from non-immunized C57 B1 mice did not protect C3H mice against BP8 tumour. Centrifugation of the supernatants at 105,000 indicated that the antigenic properties of the lysolecithin supernatant might be due to subcellular particles which were not found in the 3 molar KCl preparations.
用BP8细胞加弗氏完全佐剂（FCA）免疫的C57 B1小鼠的100万个淋巴结细胞（LNC），可保护C3H小鼠免受20小时后腹腔注射BP8所致的致命攻击。用溶血卵磷脂（1.5μg/10个细胞）处理过的BP8细胞的上清液免疫的C57 B1小鼠的LNC也能提供同样良好的保护。用溶血卵磷脂处理后的BP8细胞在抗原效力上与未处理的BP8细胞相当。用3摩尔氯化钾从BP8细胞制备的无细胞制剂的抗原效力存疑。用C3H肝脏和脾脏组织加FCA免疫的C57 B1小鼠的LNC没有保护作用，未免疫的C57 B1小鼠的LNC也不能保护C3H小鼠免受BP8肿瘤侵害。以105,000离心上清液表明，溶血卵磷脂上清液的抗原特性可能归因于在3摩尔氯化钾制剂中未发现的亚细胞颗粒。
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