Reisfeld R A, Pellegrino M A, Kahan B D
Science. 1971 Jun 11;172(3988):1134-6. doi: 10.1126/science.172.3988.1134.
Extraction of cultured human lymphoid cells with hypertonic salt solutions (3 molar potassium chloride) resulted in high recoveries of membrane-associated histocompatibility (HL-A) antigens in soluble form with potent activity and marked immunologic specificity. The active principle was purified by preparative acrylamide-gel electrophoresis. Application of the hypertonic salt extraction method is now yielding sufficient HL-A antigen to begin the elucidation of the molecular basis of transplantation individuality.
用高渗盐溶液（3摩尔氯化钾）提取培养的人淋巴细胞，可使膜相关组织相容性（HL-A）抗原以可溶性形式高回收率地得到，且具有高效活性和显著的免疫特异性。活性成分通过制备性丙烯酰胺凝胶电泳进行纯化。高渗盐提取法的应用目前正在产生足够的HL-A抗原，以便开始阐明移植个体性的分子基础。
