Terner J, Hsieh C L, Burns A R, El-Sayed M A
Biochemistry. 1979 Aug 7;18(16):3629-34. doi: 10.1021/bi00583a030.
The resonance Raman spectrum of photolyzed bacteriorhodopsin under conditions known to increase the concentration of the bO640 intermediate in both H2O and D2O is presented. By use of computer subtraction techniques and a knowledge of the Raman spectra of the unphotolyzed bacteriorhodopsin as well as the other intermediates in the cycle, a qualitative spectrum of bO640 is determined. The shift of a band at 1630 cm-1 in H2O to 1616 cm-1 in D2O suggests that the Schiff base of bO640 is protonated. Additional bands at 947, 965, and 992 cm-1 that appear only in D2O suspensions confirm that a proton is coupled to the retinal chromophore of bO640. The reprotonation of the Schiff base thus occurs during the bM412 to bO640 step. The fingerprint region, sensitive to the isomeric configuration of the retinal chromophore of bO640, is dissimilar to the fingerprint regions of published model compounds and other forms of bacteriorhodopsin.
本文给出了在已知能增加bO640中间体在H2O和D2O中浓度的条件下，光解细菌视紫红质的共振拉曼光谱。通过计算机减法技术以及对未光解细菌视紫红质和循环中其他中间体拉曼光谱的了解，确定了bO640的定性光谱。H2O中1630 cm-1处的一条谱带在D2O中移至1616 cm-1，这表明bO640的席夫碱被质子化。仅在D2O悬浮液中出现的947、965和992 cm-1处的其他谱带证实，一个质子与bO640的视黄醛发色团耦合。因此，席夫碱的再质子化发生在bM412到bO640的步骤中。bO640视黄醛发色团的异构体构型敏感的指纹区与已发表的模型化合物和细菌视紫红质其他形式的指纹区不同。
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