毫秒时间尺度上细菌视紫红质的时间分辨共振拉曼光谱
Time-resolved resonance Raman spectroscopy of bacteriorhodopsin on the millisecond timescale.
作者信息
Terner J, Campion A, El-Sayed M A
出版信息
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5212-6. doi: 10.1073/pnas.74.12.5212.
Abstract
A simple technique is described that uses a continuous wave laser with electromechanical modulation to obtain time-resolved Raman spectra of transient species on the millisecond timescale. The time behavior of the vibrational bands of the intermediates involved in the proton pumping of bacteriorhodopsin is determined. From these results, along with resonance enhancement and power dependence studies, the bands that appear in the continuous wave Raman spectrum of bacteriorhodopsin can be assigned to three intermediates in the photochemical cycle of bacteriorhodopsin, bR570, bL550, and bM412. The Raman spectra of bR570 and bM412 are compared with published spectra of model Schiff bases of all-trans and 13-cis retinal.
摘要
本文描述了一种简单的技术，该技术使用带有机电调制的连续波激光，以获取毫秒时间尺度上瞬态物种的时间分辨拉曼光谱。确定了参与细菌视紫红质质子泵浦过程的中间体振动带的时间行为。根据这些结果，以及共振增强和功率依赖性研究，细菌视紫红质连续波拉曼光谱中出现的谱带可归属于细菌视紫红质光化学循环中的三种中间体，即bR570、bL550和bM412。将bR570和bM412的拉曼光谱与已发表的全反式和13-顺式视黄醛模型席夫碱的光谱进行了比较。
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