辣根过氧化物酶催化视黄酸的氧化脱羧反应。

The oxidation and decarboxylation of retinoic acid by horseradish peroxidase.

作者信息

McKenzie R M, Nelson E C

出版信息

Biochim Biophys Acta. 1979 Jul 27;574(1):1-7. doi: 10.1016/0005-2760(79)90077-8.

Abstract

The decarboxylation of retinoic acid by horseradish peroxidase was investigated. A marked increase in the yield of products was obtained. However, the data indicated the reaction was a nonenzymatic, heme catalyzed peroxidation. Previously reported requirements for phosphate, oxygen and ferrous ion were eliminated when hydrogen peroxide was provided. Peroxide also eliminated the EDTA and cyanide induced inhibition of the phosphate dependent system. In the presence of hydrogen peroxide, horseradish peroxidase was not essential to the reaction; heme equivalent amounts of hemoglobin decarboxylated retinoic acid with equal facility. However, hemoglobin was ineffective in the absence of hydrogen peroxide. Attainment of 50--60% decarboxylation represented complete utilization of the available retinoic acid. Thus the products of the reaction can be divided into two groups, products of retinoic acid oxidation and products of an oxidative decarboxylation of retinoic acid.

摘要

研究了辣根过氧化物酶催化视黄酸的脱羧反应。产物产率显著提高。然而，数据表明该反应是一种非酶促的、血红素催化的过氧化反应。当提供过氧化氢时，先前报道的对磷酸盐、氧气和亚铁离子的需求被消除。过氧化物还消除了EDTA和氰化物对磷酸盐依赖系统的抑制作用。在过氧化氢存在的情况下，辣根过氧化物酶对反应不是必需的；等量血红素的血红蛋白能同样容易地使视黄酸脱羧。然而，在没有过氧化氢的情况下，血红蛋白没有效果。达到50%-60%的脱羧率代表可用视黄酸被完全利用。因此，反应产物可分为两组，即视黄酸氧化产物和视黄酸氧化脱羧产物。