兔肾前列腺素9-酮还原酶对前列腺素E1及前列腺素A1谷胱甘肽共轭物（谷胱甘肽-前列腺素A1）的代谢作用

Metabolism of prostaglandin E1 and of glutathione conjugate of prostaglandin A1 (GSH-prostaglandin A1) by prostaglandin 9-ketoreductase from rabbit kidney.

作者信息

Toft B S, Hansen H S

出版信息

Biochim Biophys Acta. 1979 Jul 27;574(1):33-8. doi: 10.1016/0005-2760(79)90081-x.

Abstract

Rabbit kidney prostaglandin 9-ketoreductase was found to metabolize the glutathione conjugate of prostaglandin A1 (GSH-prostaglandin A1). Apparent Km (GSH-prostaglandin A1) 13 microM and apparent Km (prostaglandin E1) 200 microM. The cytosolic preparation was subjected to gelfiltration and isoelectric focusing, which revealed that metabolism of prostaglandin E1 and GSH-prostaglandin A1 occurs by means of the same fractions. Furthermore, prostaglandin E1 and GSH-prostaglandin A1 are competitive inhibitors of the enzyme, when GSH-prostaglandin A1 and prostaglandin E1 are tested as substrates, respectively. It si concluded, that GSH-prostaglandin A1 is a much better substrate for prostaglandin 9-ketoreductase from rabbit kidney than is prostaglandin E1.

摘要

已发现兔肾前列腺素9-酮还原酶可代谢前列腺素A1的谷胱甘肽共轭物（谷胱甘肽-前列腺素A1）。表观米氏常数（谷胱甘肽-前列腺素A1）为13微摩尔，表观米氏常数（前列腺素E1）为200微摩尔。对胞质提取物进行凝胶过滤和等电聚焦，结果表明前列腺素E1和谷胱甘肽-前列腺素A1的代谢是通过相同的组分进行的。此外，当分别将谷胱甘肽-前列腺素A1和前列腺素E1作为底物进行测试时，前列腺素E1和谷胱甘肽-前列腺素A1是该酶的竞争性抑制剂。得出的结论是，与前列腺素E1相比，谷胱甘肽-前列腺素A1是兔肾前列腺素9-酮还原酶更好的底物。