Duffield A M, Duffield P H, Birkett D J, Kennedy M, Wade D N
Biomed Mass Spectrom. 1979 May;6(5):208-11. doi: 10.1002/bms.1200060508.
A quantitative method has been developed to measure plasma concentrations of warfarin and warfarin alcohol. The analytical procedure uses deuterated analogues as internal standards, and the technique of selected ion monitoring following gas chromatography methane chemical ionization mass spectrometry of the 4'-methyl ethers of warfarin and warfarin alcohol. Concentrations of warfarin and warfarin alcohol have been measured in plasma samples from 43 patients maintained on chronic warfarin therapy and compared with the 'apparent warfarin' concentration as measured by a fluorometric procedure. The study demonstrated a high degree of correlation between the gas chromatography mass spectrometric derived sum of the individual concentrations of warfarin and warfarin alcohol, and the 'apparent warfarin' concentration determined from a spectrofluorometric assay.
已开发出一种定量方法来测定华法林和华法林醇的血浆浓度。该分析程序使用氘代类似物作为内标，并采用气相色谱-甲烷化学电离质谱法对华法林和华法林醇的4'-甲基醚进行选择离子监测技术。已对43例接受慢性华法林治疗患者的血浆样本测定了华法林和华法林醇的浓度，并与通过荧光法测定的“表观华法林”浓度进行了比较。该研究表明，气相色谱-质谱法得出的华法林和华法林醇各自浓度之和与荧光分光光度法测定的“表观华法林”浓度之间存在高度相关性。
