Kopelovich L
Proc Soc Exp Biol Med. 1975 Feb;148(2):410-3.
Antisera against rat liver aspartate aminotransferase (EC 2.6.1.1) isozymes were used to study the activity and immunologic pattern of these isozymes in the livers of the rat, mouse, hamster, gerbil and in Ehrlich ascites cells. A double immunodiffusion precipitin test and immunoelectrophoresis showed that, except for the gerbil, there was a pattern of identity of AAT isozymes in the presence of either the antianionic or the anticationic antisera. Although gerbil AAT isozymes are immunochemically different from those of the other rodents studied, they were inactivated by the respective antiserum in a manner similar to that observed with the other species. This may suggest that antigenic determinants at the catalytic site of each of the liver aspartate aminotransferase isozymes are least likely to change throughout the evolutionary process.
利用抗大鼠肝脏天冬氨酸氨基转移酶（EC 2.6.1.1）同工酶的抗血清，研究了这些同工酶在大鼠、小鼠、仓鼠、沙鼠肝脏以及艾氏腹水癌细胞中的活性和免疫模式。双向免疫扩散沉淀试验和免疫电泳表明，除沙鼠外，在抗阴离子或抗阳离子抗血清存在的情况下，天冬氨酸氨基转移酶同工酶具有相同的模式。虽然沙鼠的天冬氨酸氨基转移酶同工酶在免疫化学上与所研究的其他啮齿动物不同，但它们被相应抗血清灭活的方式与其他物种相似。这可能表明，在整个进化过程中，每种肝脏天冬氨酸氨基转移酶同工酶催化位点的抗原决定簇最不可能发生变化。
