兔肝脏天冬氨酸氨基转移酶同工酶。纯化及性质

Aspartate aminotransferase isozymes from rabbit liver. Purification and properties.

作者信息

Kuramitsu S, Inoue K, Kondo K, Aki K, Kagamiyama H

出版信息

J Biochem. 1985 May;97(5):1337-45. doi: 10.1093/oxfordjournals.jbchem.a135186.

DOI:10.1093/oxfordjournals.jbchem.a135186

Abstract

Cytosolic and mitochondrial isozymes of aspartate aminotransferase (L-aspartate:2-oxoglutarate aminotransferase [EC 2.6.1.1] ) were purified to homogeneity from rabbit liver. The rabbit liver isozymes were closely similar to the corresponding isozymes from other sources, including human heart, pig heart, chicken heart, and rat liver, in their molecular weights, absorption spectra, amino acid compositions, isoelectric points, and Michaelis constants for the substrates. The NH2-terminal amino acid sequences of rabbit liver isozymes were identified up to 30 residues, and showed some differences from those of the corresponding isozymes obtained from other animals so far studied.

摘要

天冬氨酸氨基转移酶（L-天冬氨酸：2-氧代戊二酸氨基转移酶[EC 2.6.1.1]）的胞质和线粒体同工酶从兔肝中纯化至同质。兔肝同工酶在分子量、吸收光谱、氨基酸组成、等电点以及底物的米氏常数方面，与来自其他来源（包括人心、猪心、鸡心和大鼠肝）的相应同工酶极为相似。兔肝同工酶的NH2末端氨基酸序列已鉴定出多达30个残基，并且与迄今为止研究的其他动物的相应同工酶的序列存在一些差异。