Donald D, Morley K D, Beck J S
Clin Exp Immunol. 1973 Jan;13(1):101-6.
Guinea-pigs were immunized with an emulsion of human thyroglobulin (Tg) and BCG vaccine in Freund's incomplete adjuvant. Peripheral blood lymphocytes binding Tg and purified protein derivative of mammalian tuberculin (PPD) labelled with I were demonstrated with an autoradiographic technique. The percentage of these cells was compared with the size of the delayed hypersensitivity (DH) skin reactions to Tg and PPD, and Tg antibody titres at intervals up to 35 days after immunization. The individual responses to Tg showed the time course of primary immune reactions although they did not parallel each other closely. By contrast, a depression of cutaneous DH to PPD was observed in spite of the presence of substantial numbers of I-PPD-binding peripheral blood lymphocytes. In non-immune guinea-pigs, approximately 2·8% of peripheral blood lymphocytes bound I-PPD whereas <0·1% bound I-Tg. The significance of antigen-binding lymphocytes in the immune response is discussed, and it is suggested that the presence of a substantial pool of antigen-binding lymphocytes in unprimed may be of importance in the induction of DH.
用人类甲状腺球蛋白（Tg）与卡介苗（BCG）在弗氏不完全佐剂中的乳剂对豚鼠进行免疫。采用放射自显影技术显示与用I标记的Tg和哺乳动物结核菌素纯化蛋白衍生物（PPD）结合的外周血淋巴细胞。将这些细胞的百分比与免疫后长达35天期间对Tg和PPD的迟发型超敏反应（DH）皮肤反应的大小以及Tg抗体滴度进行比较。对Tg的个体反应显示了初次免疫反应的时间进程，尽管它们彼此之间并非紧密平行。相比之下，尽管存在大量结合I-PPD的外周血淋巴细胞，但仍观察到对PPD的皮肤DH反应受到抑制。在未免疫的豚鼠中，约2.8%的外周血淋巴细胞结合I-PPD，而结合I-Tg的淋巴细胞<0.1%。讨论了抗原结合淋巴细胞在免疫反应中的意义，并提出在未致敏动物中存在大量抗原结合淋巴细胞库可能对诱导DH很重要。
