在缺乏组蛋白H1的染色质亚基（或单核小体）中鉴定非组蛋白染色质蛋白。

Identification of nonhistone chromatin proteins in chromatin subunits (or mononucleosomes) devoid of histone H1.

作者信息

Chan P K, Liew C C

出版信息

Can J Biochem. 1979 Jun;57(6):666-72. doi: 10.1139/o79-084.

Abstract

Rat liver chromatin was digested by micrococcal nuclease. Chromatin subunits (or mononucleosomes) were isolated by sucrose density gradient and subsequently fractionated by 6% polyacrylamide gel electrophoresis into two major components. One component (MN1) of the mononucleosomes had a higher mobility, contained histones H2A, H2B, H3, H4, and shorter DNA fragments (140 base pairs) while the other (MN2) contained all five histones and longer DNA fragments (180 base pairs). Both submononucleosomes (MN1 and MN2) were found to contain nonhistone chromatin proteins (NHCP). By electrophoresis in 15% sodium dodecyl sulfate-polyacrylamide gel, 9 and 11 major fractions of NHCP were identified in the submononucleosomes MN1 and MN2, respectively. It was also observed that treatment of mononucleosomes with 0.6 M NaCl removes most of these NHCP and histone H1 except for two major NHCP which remain in the core particles.

摘要

用微球菌核酸酶消化大鼠肝脏染色质。通过蔗糖密度梯度分离染色质亚基（或单核小体），随后通过6%聚丙烯酰胺凝胶电泳将其分离为两个主要成分。单核小体的一个成分（MN1）迁移率较高，包含组蛋白H2A、H2B、H3、H4以及较短的DNA片段（140个碱基对），而另一个成分（MN2）包含所有五种组蛋白以及较长的DNA片段（180个碱基对）。发现两种亚单核小体（MN1和MN2）均含有非组蛋白染色质蛋白（NHCP）。通过在15%十二烷基硫酸钠-聚丙烯酰胺凝胶中进行电泳，分别在亚单核小体MN1和MN2中鉴定出9个和11个主要的NHCP组分。还观察到，用0.6 M NaCl处理单核小体可去除大部分这些NHCP和组蛋白H1，但有两个主要的NHCP保留在核心颗粒中。