用QFQ技术检测的人类3号和4号染色体着丝粒异态性频率：能否通过RFA技术识别？

Frequencies of centromeric heteromorphisms of human chromosomes 3 and 4 as detected by QFQ technique: can they be identified by RFA technique?

作者信息

Verma R S, Dosik H

出版信息

Can J Genet Cytol. 1979 Mar;21(1):109-13. doi: 10.1139/g79-014.

Abstract

One hundred normal Caucasians were studied by sequential QFQ and RFA banding techniques in order to estimate the type and frequency of heteromorphisms in the centromeric regions of chromosome 3 and 4. Intensity variants were classified into 1 of 5 levels of QFQ banding. QFQ intensity heteromorphisms (greater than or equal to level 3) for chromosomes 3 and 4 were 62 and 15 percent respectively. The interrelationship between QFQ and RFA variants were also examined. When the centromere was brilliant by QFQ, it was found that it was deep red by RFA; when it was pale by QFQ, it was light red by RFA. Neverthless, a blind coded study could not pick up these color variants by RFA. QFQ banding showed variations of the centromeric regions of chromosomes 3 and 4 while RFA banding failed to demonstrate it. It was concluded that QFQ is the most useful technique in detecting the different intensity levels in the centromeric regions of chromsomes 3 and 4.

摘要

为了估计3号和4号染色体着丝粒区域异态性的类型和频率，采用连续QFQ和RFA显带技术对100名正常白种人进行了研究。强度变异被分为QFQ显带的5个水平之一。3号和4号染色体的QFQ强度异态性（大于或等于3级）分别为62%和15%。还研究了QFQ和RFA变异之间的相互关系。当QFQ显示着丝粒明亮时，发现RFA显示为深红色；当QFQ显示着丝粒淡染时，RFA显示为浅红色。然而，一项盲编码研究未能通过RFA识别出这些颜色变异。QFQ显带显示了3号和4号染色体着丝粒区域的变异，而RFA显带未能显示。得出的结论是，QFQ是检测3号和4号染色体着丝粒区域不同强度水平的最有用技术。