Partanen M, Hervonen A, Vaalasti A, Kanerva L, Hervonen H
Cell Tissue Res. 1979 Jul 17;199(3):373-86. doi: 10.1007/BF00236076.
The vacuolated neurons (VN) of the main hypogastric ganglion of the male rat were studied using the formaldehyde-induced fluorescence (FIF) method for the histochemical demonstration of catecholamines. Microspectrofluorimetry was performed to identify the fluorophores and to quantify the FIF. The thiocholine method (Koelle-Gomori) was used to demonstrate acetylcholinesterase activity. The fine structure of the VN was studied using glutaraldehyde/OsO4 fixation. (1) In the untreated adult male rat VN represent only a small population of the total number of hypogastric neurons (0.8--1.2%). The vacuoles are similar to those of the VN from the corresponding female ganglion. (2) The VN are considered to be adrenergic due to the nature of their fluorophore, indicating a primary catecholamine. (3) The first VN appear in the hypogastric ganglia at the age of 7 weeks. After testosterone administration to young rats, VN are found at the age of 4 weeks. (4) The basic fine structure of the VN is similar to that of other ordinary neurons of the hypogastric ganglia. (5) The content of the vacuoles could not be identified. (6) Indications of degeneration were not observed in the VN. (7) The VN are interpreted as being a functional stage of the "short" adrenergic neurons, which are under the control of steroid hormones. (8) Fifteen months after castration, no VN could be found in the hypogastric ganglia, while their number was normal in the corresponding control animals.
采用甲醛诱导荧光（FIF）法对雄性大鼠主腹下神经节的空泡化神经元（VN）进行了研究，以进行儿茶酚胺的组织化学显示。进行了显微分光荧光测定以鉴定荧光团并定量FIF。采用硫代胆碱法（Koelle-Gomori）显示乙酰胆碱酯酶活性。使用戊二醛/四氧化锇固定法研究了VN的精细结构。（1）在未处理的成年雄性大鼠中，VN仅占腹下神经元总数的一小部分（0.8 - 1.2%）。这些空泡与相应雌性神经节中VN的空泡相似。（2）由于其荧光团的性质，VN被认为是肾上腺素能的，表明存在主要的儿茶酚胺。（3）第一批VN出现在7周龄的腹下神经节中。对幼鼠给予睾酮后，4周龄时可发现VN。（4）VN的基本精细结构与腹下神经节的其他普通神经元相似。（5）空泡的内容物无法确定。（6）在VN中未观察到退变迹象。（7）VN被解释为“短”肾上腺素能神经元的一个功能阶段，受类固醇激素控制。（8）去势15个月后，在腹下神经节中未发现VN，而相应对照动物中的VN数量正常。
