Lee J
Genetics. 1973 Oct;75(2):259-64. doi: 10.1093/genetics/75.2.259.
Two strains of cultivated Gossypium barbadense L., Sea Island AS-2 and Pima S-4, were used to study the effects of alleles at two loci on the production and/or storage of gossypol in mature embryos. The normal alleles, Gl(2) and Gl(3), are "native" to G. barbadense, whereas the mutant alleles, gl(2) and gl(3), were introduced from Gossypium hirsutum L. through backcrossing. Each strain was grown in three replications per trial, and one, Sea Island AS-2, was grown in three environments. Each experiment consisted of all possible crosses, including reciprocals, of the four true-breeding genotypes, plus parents. Additive effects accounted for more than 90% of the total genetic variance for seed gossypol level in all trials. Epistatic effects, though small, were frequently significant. In G. barbadense Gl(2) and Gl(3) were associated with the production of similar amounts of gossypol, whereas previous trials with cultivated varieties of G. hirsutum showed that Gl(2) was more than twice as expressive as Gl(3). The greater average productivity of seed gossypol in cultivated G. barbadense, as compared with G. hirsutum, was attributed to greater activity at the Gl(3) locus in the former species.
利用两株栽培的海岛棉（Gossypium barbadense L.）品种，海岛AS - 2和皮马S - 4，研究两个位点的等位基因对成熟胚中棉酚产生和/或储存的影响。正常等位基因Gl(2)和Gl(3)是海岛棉“固有的”，而突变等位基因gl(2)和gl(3)是通过回交从陆地棉（Gossypium hirsutum L.）引入的。每个品种每次试验种植三个重复，其中一个品种，海岛AS - 2，在三种环境下种植。每个实验包括四个纯合基因型（包括正反交）以及亲本的所有可能杂交组合。在所有试验中，加性效应占种子棉酚水平总遗传变异的90%以上。上位性效应虽然较小，但常常显著。在海岛棉中，Gl(2)和Gl(,)与棉酚产量相近，而之前对陆地棉栽培品种的试验表明，Gl(2)的表达量是Gl(3)的两倍多。与陆地棉相比，栽培海岛棉种子棉酚的平均产量更高，这归因于前者在Gl(3)位点的活性更高。
