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血浆总胆固醇的自动测定:一种血清校准技术。

Automated determination of total plasma cholesterol: a serum calibration technique.

作者信息

Bachorik P S, Wood P D, Williams J, Kuchmak M, Ahmed S, Lippel K, Albers J

出版信息

Clin Chim Acta. 1979 Aug 15;96(1-2):145-53. doi: 10.1016/0009-8981(79)90064-0.

DOI:10.1016/0009-8981(79)90064-0
PMID:476955
Abstract

The automated (AutoAnalyzer II) determination of cholesterol in nine serum pools in the concentration range 3.465-8.871 mmol/l, gave results that were approximately 10% higher than reference values when the analyses were based on unesterified cholesterol standards containing the same amount of water as the sample extracts (1963 analyses in 12 laboratories during a 12 month period; automated value = 0.032 + 1.10 X (Reference value)). A serum calibration procedure was successful ilues, and was equally effective in correcting the values observed for aliquots of 368 fresh-frozen plasma samples analyzed in each of the 12 laboratories during a 38 month period.

摘要

使用自动分析仪II对九个血清池(胆固醇浓度范围为3.465 - 8.871 mmol/l)进行胆固醇测定时,当分析基于含有与样品提取物相同水量的游离胆固醇标准品时,所得结果比参考值约高10%(在12个月期间12个实验室进行了1963次分析;自动分析值 = 0.032 + 1.10×(参考值))。一种血清校准程序在38个月期间对12个实验室各自分析的368份新鲜冷冻血浆样品的等分试样进行校正时很成功,且同样有效。

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