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参与嘌呤核苷酸从头生物合成的一种酶对，即磷酸核糖氨基咪唑羧化酶和磷酸核糖氨基咪唑琥珀酰胺羧酰胺合成酶的纯化及性质

Purification and properties of an enzyme duet, phosphoribosylaminoimidazole carboxylase and phosphoribosylaminoimidazolesuccinocarboxamide synthetase, involved in the biosynthesis of purine nucleotides de novo.

作者信息

Patey C A, Shaw G

出版信息

Biochem J. 1973 Nov;135(3):543-5. doi: 10.1042/bj1350543.

DOI:10.1042/bj1350543
PMID:4772278
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1165858/
Abstract

Purification and some properties of an adjacent pair of enzymes in the biosynthetic pathway to AMP, phosphoribosylaminoimidazole carboxylase (EC 4.1.1.21) and phosphoribosylaminoimidazolesuccinocarboxamide synthetase (EC 6.3.2.6), are described. The enzymes form a duet, which may be regarded as a single molecule with a dual function, or as two very similar substances.

摘要

本文描述了腺苷酸生物合成途径中相邻的一对酶——磷酸核糖氨基咪唑羧化酶（EC 4.1.1.21）和磷酸核糖氨基咪唑琥珀酰胺羧酰胺合成酶（EC 6.3.2.6）的纯化及某些特性。这两种酶形成了一个二重奏，它可以被视为具有双重功能的单个分子，或者是两种非常相似的物质。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d78/1165858/69908c93c2ef/biochemj00597-0172-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d78/1165858/69908c93c2ef/biochemj00597-0172-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d78/1165858/69908c93c2ef/biochemj00597-0172-a.jpg

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Biosynthesis of the purines. XVIII. 5-Amino-1-ribosyl-4-imidazolecarboxamide 5'-phosphate transformylase and inosinicase.嘌呤的生物合成。十八。5-氨基-1-核糖基-4-咪唑甲酰胺5'-磷酸转甲酰酶和肌苷酶。
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通过在酿酒酵母中对ade2 - 101突变体进行互补作用克隆和测序编码嘌呤途径多功能多肽的人类cDNA。
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Molecular genetic analysis of Saccharomyces cerevisiae C1-tetrahydrofolate synthase mutants reveals a noncatalytic function of the ADE3 gene product and an additional folate-dependent enzyme.酿酒酵母C1-四氢叶酸合酶突变体的分子遗传学分析揭示了ADE3基因产物的非催化功能以及另一种叶酸依赖性酶。
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6
DNA sequence of the purC gene encoding 5'-phosphoribosyl-5-aminoimidazole-4-N-succinocarboxamide synthetase and organization of the dapA-purC region of Escherichia coli K-12.编码5'-磷酸核糖基-5-氨基咪唑-4-N-琥珀酰胺羧酰胺合成酶的purC基因的DNA序列及大肠杆菌K-12的dapA-purC区域的组织架构
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