• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过在酿酒酵母中对ade2 - 101突变体进行互补作用克隆和测序编码嘌呤途径多功能多肽的人类cDNA。

Cloning and sequencing of a human cDNA coding for a multifunctional polypeptide of the purine pathway by complementation of the ade2-101 mutant in Saccharomyces cerevisiae.

作者信息

Minet M, Lacroute F

机构信息

Centre de Génétique Moléculaire, C.N.R.S., Gif sur Yvette, France.

出版信息

Curr Genet. 1990 Nov;18(4):287-91. doi: 10.1007/BF00318209.

DOI:10.1007/BF00318209
PMID:2253271
Abstract

A HeLa cell cDNA library on a yeast expression vector was used to complement auxotrophic markers of Saccharomyces cerevisiae. Clones complementing the ade2-101 mutation harbor a 1.5 kb poly(A)+ tailed insert with a 425 amino acid open reading frame hybridizing with two human mRNAs of 1.5 kb and 3.1 kb. Its 5' half is homologous to Bacillus subtilis SAICAR synthetase (E.C.6.3.2.6.) and its 3' terminal half corresponds to the catalytic subunit of Escherichia coli and B. subtilis AIR carboxylase (E.C.4.1.1.21). In agreement with these homologies, pADE2H1 clones complement both ade1 and ade2 mutants of S. cerevisiae, as was also recently reported for a 3.1 kb cDNA isolated from human hepatocytes.

摘要

利用酵母表达载体上的HeLa细胞cDNA文库来互补酿酒酵母的营养缺陷型标记。互补ade2-101突变的克隆含有一个1.5 kb的聚腺苷酸加尾插入片段，其开放阅读框为425个氨基酸，可与两条分别为1.5 kb和3.1 kb的人mRNA杂交。其5'端一半与枯草芽孢杆菌SAICAR合成酶（E.C.6.3.2.6.）同源，3'端一半与大肠杆菌和枯草芽孢杆菌AIR羧化酶的催化亚基（E.C.4.1.1.21）对应。与这些同源性一致，pADE2H1克隆可互补酿酒酵母的ade1和ade2突变体，最近从人肝细胞中分离出的一个3.1 kb cDNA也有此报道。

相似文献

1
Cloning and sequencing of a human cDNA coding for a multifunctional polypeptide of the purine pathway by complementation of the ade2-101 mutant in Saccharomyces cerevisiae.通过在酿酒酵母中对ade2 - 101突变体进行互补作用克隆和测序编码嘌呤途径多功能多肽的人类cDNA。
Curr Genet. 1990 Nov;18(4):287-91. doi: 10.1007/BF00318209.
2
Cloning of three human multifunctional de novo purine biosynthetic genes by functional complementation of yeast mutations.通过酵母突变体的功能互补克隆三个人类多功能从头嘌呤生物合成基因。
Proc Natl Acad Sci U S A. 1990 Apr;87(8):2916-20. doi: 10.1073/pnas.87.8.2916.
3
The 5-aminoimidazole ribonucleotide-carboxylase structural gene of the methylotrophic yeast Pichia methanolica: cloning, sequencing and homology analysis.甲基营养型酵母甲醇毕赤酵母5-氨基咪唑核糖核苷酸羧化酶结构基因：克隆、测序及同源性分析。
Yeast. 1993 Nov;9(11):1251-8. doi: 10.1002/yea.320091112.
4
Cloning of a chicken liver cDNA encoding 5-aminoimidazole ribonucleotide carboxylase and 5-aminoimidazole-4-N-succinocarboxamide ribonucleotide synthetase by functional complementation of Escherichia coli pur mutants.通过大肠杆菌嘌呤突变体的功能互补克隆编码5-氨基咪唑核糖核苷酸羧化酶和5-氨基咪唑-4-N-琥珀酰胺羧酰胺核糖核苷酸合成酶的鸡肝cDNA
Proc Natl Acad Sci U S A. 1990 Apr;87(8):3097-101. doi: 10.1073/pnas.87.8.3097.
5
[Cloning and physical mapping of the ADE1 gene of the yeast Saccharomyces cerevisiae].[酿酒酵母ADE1基因的克隆与物理图谱构建]
Genetika. 1986 Apr;22(4):549-56.
6
Cloning and sequence analysis of the Candida boidinii ADE2 gene.博伊丁假丝酵母ADE2基因的克隆与序列分析。
Yeast. 2000 Jul;16(10):953-7. doi: 10.1002/1097-0061(200007)16:10<953::AID-YEA590>3.0.CO;2-D.
7
Molecular cloning of the Candida maltosa ADE1 gene.麦芽糖假丝酵母ADE1基因的分子克隆
Gene. 1991 Oct 30;107(1):161-4. doi: 10.1016/0378-1119(91)90311-x.
8
Primary structure of the ADE1 gene from Candida utilis.产朊假丝酵母ADE1基因的一级结构
Biosci Biotechnol Biochem. 1994 Jan;58(1):208-10. doi: 10.1271/bbb.58.208.
9
[Cloning of the ADE2 gene of Saccharomyces cerevisiae and localization of the ARS-sequence].[酿酒酵母ADE2基因的克隆及自主复制序列（ARS）的定位]
Genetika. 1987 Jul;23(7):1141-8.
10
The Saccharomyces cerevisiae ADE1 gene: structure, overexpression and possible regulation by general amino acid control.酿酒酵母ADE1基因：结构、过表达及可能受一般氨基酸调控的情况
Gene. 1991 Dec 20;109(1):143-7. doi: 10.1016/0378-1119(91)90600-g.

引用本文的文献

1
Birch PR-10c is induced by factors causing oxidative stress but appears not to confer tolerance to these agents.桦木PR - 10c由引起氧化应激的因素诱导产生，但似乎并未赋予对这些因素的耐受性。
New Phytol. 2002 Sep;155(3):381-391. doi: 10.1046/j.1469-8137.2002.00481.x.
2
An integrated biochemistry and genetics outreach program designed for elementary school students.一个面向小学生的综合生物化学和遗传学外展计划。
Genetics. 2012 Feb;190(2):305-15. doi: 10.1534/genetics.111.135285. Epub 2011 Nov 30.
3
Identification of a novel human nuclear-encoded mitochondrial poly(A) polymerase.

本文引用的文献

1
Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.通过在大肠杆菌中进行DNA融合和克隆分析基因控制信号。
J Mol Biol. 1980 Apr;138(2):179-207. doi: 10.1016/0022-2836(80)90283-1.
2
Nucleotide sequence of the yeast plasmid.酵母质粒的核苷酸序列。
Nature. 1980 Aug 28;286(5776):860-5. doi: 10.1038/286860a0.
3
The sequence 5'-AAUAAA-3'forms parts of the recognition site for polyadenylation of late SV40 mRNAs.序列5'-AAUAAA-3'构成了猴病毒40（SV40）晚期mRNA聚腺苷酸化识别位点的一部分。
一种新型人类核编码线粒体多聚腺苷酸聚合酶的鉴定。
Nucleic Acids Res. 2004 Nov 16;32(20):6001-14. doi: 10.1093/nar/gkh923. Print 2004.
4
The C-terminal region of mitochondrial single-subunit RNA polymerases contains species-specific determinants for maintenance of intact mitochondrial genomes.线粒体单亚基RNA聚合酶的C末端区域包含用于维持完整线粒体基因组的物种特异性决定因素。
Mol Biol Cell. 2002 Jul;13(7):2245-55. doi: 10.1091/mbc.01-07-0359.
5
Enhanced copper tolerance in Silene vulgaris (Moench) Garcke populations from copper mines is associated with increased transcript levels of a 2b-type metallothionein gene.来自铜矿的麦瓶草种群中增强的铜耐受性与一种2b型金属硫蛋白基因转录水平的增加有关。
Plant Physiol. 2001 Aug;126(4):1519-26. doi: 10.1104/pp.126.4.1519.
6
Development of a Saccharomyces cerevisiae strain with enhanced resistance to phenolic fermentation inhibitors in lignocellulose hydrolysates by heterologous expression of laccase.通过漆酶的异源表达开发一种对木质纤维素水解产物中酚类发酵抑制剂具有增强抗性的酿酒酵母菌株。
Appl Environ Microbiol. 2001 Mar;67(3):1163-70. doi: 10.1128/AEM.67.3.1163-1170.2001.
7
The Drosophila melanogaster ade5 gene encodes a bifunctional enzyme for two steps in the de novo purine synthesis pathway.果蝇的ade5基因编码一种双功能酶，参与从头嘌呤合成途径中的两个步骤。
Genetics. 2000 Mar;154(3):1239-53. doi: 10.1093/genetics/154.3.1239.
8
Primary autosomal recessive microcephaly (MCPH1) maps to chromosome 8p22-pter.原发性常染色体隐性小头畸形（MCPH1）定位于8号染色体短臂22区至末端。
Am J Hum Genet. 1998 Aug;63(2):541-6. doi: 10.1086/301966.
9
A general suppressor of RNA polymerase I, II and III mutations in Saccharomyces cerevisiae.酿酒酵母中RNA聚合酶I、II和III突变的一般抑制因子。
Mol Gen Genet. 1993 May;239(1-2):169-76. doi: 10.1007/BF00281615.
10
Identification of a purC gene from Streptococcus pneumoniae.从肺炎链球菌中鉴定出一个purC基因。
J Bacteriol. 1993 Oct;175(19):6364-7. doi: 10.1128/jb.175.19.6364-6367.1993.
Cell. 1981 Apr;24(1):251-60. doi: 10.1016/0092-8674(81)90521-3.
4
Comparison of the complementation and genetic maps of closely linked nonallelic markers on linkage group I of Neurospora crassa.粗糙脉孢菌连锁群I上紧密连锁的非等位标记的互补图谱与遗传图谱的比较。
Mutat Res. 1969 Jul-Aug;8(1):43-50. doi: 10.1016/0027-5107(69)90139-0.
5
Interallelic complementation at the ad-2 locus of Saccharomyces cerevisiae.
Heredity (Edinb). 1966 Feb;21(1):121-30. doi: 10.1038/hdy.1966.80.
6
Purification and properties of an enzyme duet, phosphoribosylaminoimidazole carboxylase and phosphoribosylaminoimidazolesuccinocarboxamide synthetase, involved in the biosynthesis of purine nucleotides de novo.参与嘌呤核苷酸从头生物合成的一种酶对，即磷酸核糖氨基咪唑羧化酶和磷酸核糖氨基咪唑琥珀酰胺羧酰胺合成酶的纯化及性质
Biochem J. 1973 Nov;135(3):543-5. doi: 10.1042/bj1350543.
7
Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2.利用互补作用克隆了裂殖酵母细胞周期控制基因cdc2的一个人类同源基因。
Nature. 1987;327(6117):31-5. doi: 10.1038/327031a0.
8
Genetic and physical analysis of the M26 recombination hotspot of Schizosaccharomyces pombe.粟酒裂殖酵母M26重组热点的遗传与物理分析。
Genetics. 1988 Jul;119(3):491-7. doi: 10.1093/genetics/119.3.491.
9
A yeast activity can substitute for the HeLa cell TATA box factor.酵母活性可以替代海拉细胞的TATA盒因子。
Nature. 1988 Jul 7;334(6177):77-80. doi: 10.1038/334077a0.
10
The human oestrogen receptor functions in yeast.人类雌激素受体在酵母中发挥作用。
Nature. 1988 Jul 7;334(6177):31-6. doi: 10.1038/334031a0.