溶壁微球菌磷酸葡萄糖变位酶中的反应性丝氨酸残基。
The reactive serine residue in phosphoglucomutase of Micrococcus lysodeikiticus.
作者信息
Milstein C P, Clarke J B, Britton H G
出版信息
Biochem J. 1973 Nov;135(3):551-3. doi: 10.1042/bj1350551.
Abstract
- Phosphoglucomutase of Micrococcus lysodeikticus was labelled at the active site by exchange with (32)P-labelled substrates of high specific radioactivity. 2. Partial acid hydrolysis gave rise to radioactive peptides; serine phosphate was identified as one of the derivatives. 3. Comparison of the other (32)P-labelled peptides with the peptides obtained from the (32)P-labelled rabbit muscle phosphoglucomutase indicates that the sequence around the reactive serine residue is identical in both enzymes.
摘要
- 通过与高比放射性的（32）P标记底物进行交换，对溶壁微球菌的磷酸葡萄糖变位酶在活性位点进行标记。2. 部分酸水解产生放射性肽；磷酸丝氨酸被鉴定为衍生物之一。3. 将其他（32）P标记肽与从（32）P标记的兔肌肉磷酸葡萄糖变位酶获得的肽进行比较，结果表明两种酶中活性丝氨酸残基周围的序列相同。
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J Biol Chem. 1957 Sep;228(1):419-31.
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