脑组织酸水解产物中的蛋白质结合磷酸丝氨酸。通过离子交换色谱法和电泳法测定（32P）磷酸丝氨酸。
Protein-bound phosphorylserine in acid hydrolysates of brain tissue. The determination of ( 32 P)phosphorylserine by ion-exchange chromatography and electrophoresis.
作者信息
Jones D A, Rodnight R
出版信息
Biochem J. 1971 Feb;121(4):597-600. doi: 10.1042/bj1210597.
Abstract
- Partial acid hydrolysates of proteins derived from cortical slices of guinea-pig brain were divided into two parts and fractionated by ion-exchange chromatography and high-voltage electrophoresis. 2. The apparent yield of protein-bound phosphorylserine by the ion-exchange method was about three times that obtained by electrophoresis. 3. The specific radioactivity of phosphorylserine isolated from (32)P-labelled slices by electrophoresis was twice that isolated by chromatography. 4. The discrepancies were found to be due to the presence of unlabelled phosphates of unknown composition in the ;phosphorylserine' fraction obtained by the ion-exchange method. 5. Electrical stimulation of slices respiring in the presence of [(32)P]phosphate increased the specific radioactivity of the total phosphate in the chromatographic ;phosphorylserine' fraction by 53+/-11%, as compared with only 19+/-5% for the phosphorylserine isolated by electrophoresis.
摘要
- 将豚鼠脑皮质切片来源的蛋白质部分酸水解产物分成两部分，通过离子交换色谱法和高压电泳进行分级分离。2. 离子交换法测定的蛋白质结合磷酸丝氨酸的表观产率约为电泳法的三倍。3. 通过电泳从（32）P标记切片中分离出的磷酸丝氨酸的比放射性是通过色谱法分离出的两倍。4. 发现差异是由于离子交换法获得的“磷酸丝氨酸”级分中存在未知组成的未标记磷酸盐。5. 在[（32）P]磷酸盐存在下对切片进行电刺激，与通过电泳分离的磷酸丝氨酸相比，色谱“磷酸丝氨酸”级分中总磷酸盐的比放射性增加了53±11%，而电泳分离的磷酸丝氨酸仅增加了19±5%。
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